Cholescintigraphy with99mTc-diethyl-IDA for the detection of rejection of auxiliary liver transplants in pigs
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In 15 pigs an auxiliary liver was transplanted and cholescintigraphy with99mTc-diethyl-IDA was performed to study the function of the livers separately. Serial scintigraphy of the recipient and donor liver was performed on days 3, 8 and 18 after transplantation and time-activity curves over 1 h after injection of the radio-tracer were generated for each liver. From these data the time at which the maximal activity was present in the liver (Tmax) and the time at which during the excretion phase half of this activity was still present in the organ (T1/2) were determined. Liver biopsy material from the recipient liver and the donor liver was obtained at (approximately) the day of one of the scintigraphic examinations and at autopsy.
In all cases no histological abnormalities were found in the recipient liver. In 4 pigs there were no histological signs of rejection of the donor liver, in which cases the values forTmax andT1/2 were normal. In 11 pigs histological signs of rejection were noticed and in all of these casesT1/2 was significantly prolonged, whereas no changes inTmax were observed. Cholescintigraphy revealed an abnormal value forT1/2 a few days before the biopsy was taken in 10 of these 11 cases. Normal functioning or the prediction of rejection of the auxiliary liver transplant in pigs correlated significantly (P<0.01) with normal or prolongedT1/2 values. It is concluded that cholescintigraphy may be helpful in establishing the diagnosis of rejection of liver transplants.
KeywordsPublic Health Nuclear Medicine Liver Transplant Normal Functioning Liver Biopsy
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