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Separation of pantothenic acid derivatives as precursors for the biosynthesis of the acetylation coenzyme by chromatography on DEAE-dellulose

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Abstract

The possibility of the chromatographic separation of pantothenic acid derivatives — 4′-phosphopantothenate, pantethine, 4′-phosphopantetheine, dephospho-CoA and CoA — on a column of DEAE-cellulose (DE-11 or Servacel DEAE 23 SH) has been studied, with their detection by radiometric, enzymatic, or spectrophotometric methods. The affinity of pantothenic acid and that of pantotheine for the ion-exchange resins used are identical. The phosphorylated derivatives of these compounds, which are eluted at higher values of a concentration gradient of lithium chloride, also have identical chromatographic characteristics. The separation of dephospho-CoA and CoA is not achieved on the chromatographic system investigated, but the elution of the fractions to free them from the retained nucleotide-containing precursors of the coenzyme can be used effectively for analytical purposes.

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Authors
  1. A. G. Moiseenok
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  2. V. A. Gurinovich
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  3. V. A. Lysenkova
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Additional information

Institute of Biochemistry, Academy of Sciences of the Belorussian SSR, Grodno. Translated from Khimiya Prirodnykh soedinenii, No. 2, pp. 258–262, March–April, 1987.

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Moiseenok, A.G., Gurinovich, V.A. & Lysenkova, V.A. Separation of pantothenic acid derivatives as precursors for the biosynthesis of the acetylation coenzyme by chromatography on DEAE-dellulose. Chem Nat Compd 23, 216–219 (1987). https://doi.org/10.1007/BF00598762

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  • DOI: https://doi.org/10.1007/BF00598762

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