Analysis of oligospirostanosides in a suspension culture of Dioscorea deltoidea cells by high-performance liquid chromatography

  • V. N. Paukov
  • I. S. Vasil'eva
  • N. N. Karasev
  • V. A. Paseshnichenko


A method has been developed for the quantitative determination of oligospirostanosides in a culture ofDioscorea deltoidea cells with the aid of HPLC on a LiChrosorb RP-18 column. Elution was performed with acetonitrile-water (50–75%) with detection at 207 nm.


Suspension Culture Lactam Vindoline Dioscin Steroid Glycoside 

Literature cited

  1. 1.
    S. L. Karanova, A. M. Nosov, V. N. Paukov, and Z. B. Shamina, The Culture of Plant Cells and Biotechnology [in Russian], Nauka, Moscow (1986), p. 83.Google Scholar
  2. 2.
    I. S. Vasil'eva, A. S. Vorob'ev, N. V. Gorskaya, A. Kh. Lipskii, K. G. Gurielidze, and V. A. Paseshnichenko, Prikl. Biokhim. Mikrobiol.,23, 692 (1986).Google Scholar
  3. 3.
    G. A. Tarakanova, V. A. Paseshnichenko, N. L. Gudskov, and R. G. Butenko, Prikl. Biokhim. Mikrobiol.,15, 106 (1979).Google Scholar
  4. 4.
    I. S. Vasil'eva and V. A. Paseshnichenko, Dokl. Akad. Nauk SSR,285, 767 (1987).Google Scholar
  5. 5.
    R. Hardman and E. A. Sofowora, Planta Med.,20, 124 (1971).PubMedCrossRefGoogle Scholar
  6. 6.
    V. A. Paseshnichenko and A. R. Guseva, Prikl. Biokhim. Mikrobiol.,11, No. 1, 94 (1975).PubMedGoogle Scholar
  7. 7.
    V. A. Paseshnichenko, A. R. Guseva, M. G. Borikhina, V. D. Shcherbukhin, and I. S. Vasil'eva, Prikl. Biokhim. Mikrobiol.,14, No. 5, 755 (1980).Google Scholar

Copyright information

© Plenum Publishing Corporation 1989

Authors and Affiliations

  • V. N. Paukov
  • I. S. Vasil'eva
  • N. N. Karasev
  • V. A. Paseshnichenko

There are no affiliations available

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