Abstract
The solubility of gossypulin and its derivatives under various conditions has been studied by turbidimetric titration. It was found that the low solubility of the protein under investigation in the neutral range of pH values at a low ionic strength may be due to the presence of phytin and gossypol. The minimum in the solubility of the gossypol-free protein shifted into the more alkaline region (pH 6.0–6.7) as compared with the initial gossypulin (with 0.6% of gossypol). The treatment of gossypulin with dilute solutions of phytin at pH 5.0 led to a shift in the pH of precipitation (pH 3.75), but in the presence of an excess of phytic acid the aggregation capacity of the protein increased and the pH of precipitation changed (pH 4.5–5.0). When Ca2+ ions were added, there was a shift of the solubility minimum into the alkaline pH range. In the presence of pectin, the pH of precipitation of all the samples studied shifted into a more acid pH range.
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Additional information
Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 359–364, May–June, 1986.
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Piyakina, G.A., Yunusov, T.S. Functional properties of “cotton protein.” III. Influence of gossypol and phytates on the solubility of gossypulin. Chem Nat Compd 22, 333–337 (1986). https://doi.org/10.1007/BF00598310
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DOI: https://doi.org/10.1007/BF00598310