Chemistry of Natural Compounds

, Volume 24, Issue 6, pp 708–712 | Cite as

Multimeric high-performance liquid chromatography of plant sterols using UV detection

  • Yu. F. Krylov
  • B. S. Krynetskii
  • B. S. Prokhorov
  • T. S. Oretskaya


A method for the multimeric high-performance liquid chromatography of plant sterols is proposed which permits the separation of compounds close in structure with similar chromatographic properties. The first stage includes the chemical modification of the sterols with the aid of a bis(p-nitrophenyl) phosphate group. The phosphotriesters formed as the result of the reaction are separated by normal-phase HPLC. The compounds isolated are treated with ammonia, as a result of which the sterol phosphotriesters are converted into the corresponding phosphodiesters. These phosphodiesters are then subjected to reversed-phase HPLC. The chromatographic separation of UV-absorbing sterol derivatives using several variants of HPLC substantially increases the resolving power of the method.


Ergosterol Phytosterol Diester Sitosterol Plant Sterol 


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Literature cited

  1. 1.
    L. Fieser and M. Fieser, Steroids, Reinhold, New York (1959) [Russian translation: Mir, Moscow (1964), p. 357].Google Scholar
  2. 2.
    W. R. Nes, “A comparison of methods for the identification of sterols,” Meth. Enzymol.,111, 3 (1985).CrossRefGoogle Scholar
  3. 3.
    S. Gereg, The Quantitative Analysis of Steroids [Russian translation], Mir, Moscow (1985), p. 281.Google Scholar
  4. 4.
    R. J. Rodriguez and L. W. Parks, “HPLC of sterols: yeast sterols,” Meth. Enzymol.,111, 37 (1985).CrossRefGoogle Scholar
  5. 5.
    F. A. Fitzpatrick and S. Siggia, Anal. Chem.,45, 2310 (1973).CrossRefGoogle Scholar
  6. 6.
    V. A. Efimov, S. V. Reverdatto, and O. G. Chakmakhcheva, Nucleic Acids Res.,10, 6675 (1982).CrossRefGoogle Scholar

Copyright information

© Plenum Publishing Corporation 1989

Authors and Affiliations

  • Yu. F. Krylov
  • B. S. Krynetskii
  • B. S. Prokhorov
  • T. S. Oretskaya

There are no affiliations available

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