Chemistry of Natural Compounds

, Volume 25, Issue 1, pp 112–115 | Cite as

Immobilization of snake venom on epoxy-activated Silokhroms

  • O. V. Ostapenko
  • V. V. Chupin
  • G. A. Serebrennikova
  • R. P. Evstigneeva
Article
  • 29 Downloads

Abstract

The production of immobilized phospholipase A2 preparations by the covalent addition of the proteins of viper venom to Silokhroms containing epoxide groups is described. The dependence of the amount of protein bound to the support on its concentration and on the amount of epoxide groups in the sorbent has been investigated. The phospholipase activity of the preparations obtained did not depend on the amount of immobilized enzyme but was determined by diffusional factors.

Keywords

Organic Chemistry Epoxide Immobilize Enzyme Snake Venom Covalent Addition 

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Literature cited

  1. 1.
    D. Lombardo and E. A. Dennis, J. Biol. Chem.,260, No. 30, 16114 (1985).Google Scholar
  2. 2.
    M. Adamich, H. F. Voss, and E. A. Dennis, Arch. Biochem. Biophys.,189, No. 2, 417 (1978).Google Scholar
  3. 3.
    U. R. Apsalon, O. G. Shamborant, and A. I. Miroshnikov, Bioorg. Khim.,3, No. 11, 1553 (1977).Google Scholar
  4. 4.
    N. G. Evstratova, O. V. Ostapenko, G. A. Serebrennikova, and R. P. Evstigneeva, Bioorg. Khim.,10, No. 6, 844 (1984).Google Scholar
  5. 5.
    M. Kh. Tadzhiev, F. Ibragimov, Kh. G. Khasanov, N. R. Dzhanbaeva, and M. M. Rakhimov, Prikl. Biokhim. Mikrobiol.,14, No. 5, 703 (1978).Google Scholar
  6. 6.
    Y. Kimura, A. Tanaka, K. Sonomoto, T. Nihira, and S. Fukui, Eur. J. Appl. Microbiol. Biotechnol.,17, No. 1, 107 (1983).Google Scholar
  7. 7.
    H. Jiro and M. Satiko, Kobunshi, High Polym. Jpn.,35, No. 6, 565 (1986).Google Scholar
  8. 8.
    Kha Zen Te, R. Akhmedzhanov, and M. M. Rakhimov, Uzb. Biol. Zh., No. 6, 3 (1985).Google Scholar
  9. 9.
    O. V. Lebedeva, I. G. Frumkina, and N. N. Ugarova, Prikl. Khim. Mikrobiol.,22, No. 5, 669 (1986).Google Scholar
  10. 10.
    G. S. Murthy and N. R. Moudgal, J. Biosci.,10, No. 3, 351 (1986).Google Scholar
  11. 11.
    N. Oda, M. Yoshida, S. Tanaka, H. Kihara, and M. Ohno, J. Biochem. (Tokyo),100, No. 6, 1551 (1986).Google Scholar
  12. 12.
    E. A. Dennis, J. Lipid Res.,14, No. 1, 152 (1973).Google Scholar
  13. 13.
    N. I. Polikarpova, Lab. Delo., No. 7, 447 (1986).Google Scholar

Copyright information

© Plenum Publishing Corporation 1989

Authors and Affiliations

  • O. V. Ostapenko
  • V. V. Chupin
  • G. A. Serebrennikova
  • R. P. Evstigneeva

There are no affiliations available

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