Chemistry of Natural Compounds

, Volume 25, Issue 1, pp 109–112 | Cite as

Quantitative determination of nucleic acids in salmonidae milt by various methods

  • V. A. Karklinya
  • I. A. Birska
  • Yu. A. Limarenko
Article
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Abstract

Procedures for the determination of total nucleic acids from ultraviolet absorption and for the separate determination of DNA and RNA by means of color reactions using the same tissue extracts have been developed. The results of the total and separate determination of the nucleic acids and the analysis of milts preserved by various methods are given. The discrepancies between the total amount of nucleic acids determined by the color reactions and by ultraviolet absorption do not exceed ±1.9%.

Keywords

Organic Chemistry Nucleic Acid Quantitative Determination Tissue Extract Color Reaction 

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Literature cited

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    FRG Patent No. 1,142,365 of Jan. 17, 1963.Google Scholar
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    Japanese Patent No. 8310 of February 2, 1971.Google Scholar
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    A. S. Spirin, Biokhimiya,23, No. 5, 655 [sic].Google Scholar
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    Z. Dische, in: Nucleic Acids (ed. E. Chargaff), [Russian translation, Moscow (1975), pp. 425 and 437].Google Scholar

Copyright information

© Plenum Publishing Corporation 1989

Authors and Affiliations

  • V. A. Karklinya
  • I. A. Birska
  • Yu. A. Limarenko

There are no affiliations available

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