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Use of Sephadex LH-20 for solid-phase synthesis of oxytocin

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Abstract

The solid-phase synthesis of oxytocin on commercial Sephadex LH-20 is described. The biological activity of the product obtained was 132 AU/mg, and its oxytocin content, according to HPLC, was 28.2%. It was shown that the amount of oxytocin and the biological activity do not depend on the degree of addition of Gly9 to the polymer matrix within the range of 0.6–1.6 mmoles of Gly9/g of resin; after regeneration, the Sephadex LH-20 can be re-used for solid-phase synthesis of oxytocin.

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Literature cited

  1. G. P. Vlasov and A. Yu. Bilibin, USSR Inventor's Certificate No. 250153, cl. C 07 c 104/52; Byull Izobret., No. 26, p. 24 (1969); G. P. Vlasov, V. A. Gusel', N. Yu. Kozhevnikova, V. N. Pavlov, N. G. Illarionova, I. B. Ditkovskaya, E. N. Krasnova, M. Yu. Dorosh, Yu. B. Moskvicheva, I. G. Denisov, and O. S. Veselkina, Bioorg. Khim.,12, No. 5, 591 (1986).

  2. G. I. Aukone, I. L. Gibiete, R. M. Stamere, and U. O. Kalei, in: Abstracts of Lectures at the Vth All-Union Conference on Methods of Obtaining and Analyzing Biochemical Preparations [in Russian], Riga (1987), p. 280.

  3. V. F. Pozdnev, Bioorg. Khim.,11, No. 5, 583 (1985); V. F. Pozdnev, N. N. Podgornova, I. K. Yakaite, and U. O. Kalei, Bioorg. Khim.,12, No. 7, 898 (1986).

    Google Scholar 

  4. L. C. Dorman, Tetrahedron Lett., No. 28, 2319 (1969).

  5. K. Sturm, R. Geiger, and W. Siedel, Swiss Patent No. 481883, cl. C 07 c 103/52 (1969).

  6. K. Sturm, R. Geiger, and W. Siedel, Swiss Patent No. 462190, cl. C 07 c 103/52 (1968).

  7. I. É. Zel'tser, A. A. Antonov, A. K. Ivanov, V. P. Pakhomov, and V. N. Karel'skii, Khim. Prir. Soedin., 892 (1987).

  8. I. A. Éskin, Principles of the Physiology of the Endocrine Glands [in Russian], Vysshaya Shkola (1975), p. 237.

  9. L. Bellamy, Infrared Spectra of Complex Molecules, 2nd edn., Wiley, New York (1958) [Russian translation, IL, Moscow (1963), pp. 258 and 319].

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Authors
  1. I. É. Zel'tser
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  2. A. A. Antonov
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  3. V. N. Karel'skii
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  4. E. A. Prikhodkina
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  5. A. M. Ovsepyan
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  6. E. E. Zhuravleva
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Additional information

All-Union Scientific-Research Institute of the Technology of Blood Substitutes and Hormone Preparations, Moscow. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 106–113, January–February, 1989.

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Zel'tser, I.É., Antonov, A.A., Karel'skii, V.N. et al. Use of Sephadex LH-20 for solid-phase synthesis of oxytocin. Chem Nat Compd 25, 94–100 (1989). https://doi.org/10.1007/BF00596710

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  • DOI: https://doi.org/10.1007/BF00596710

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