Summary
Freeze etching observations of a case of xanthelasma palpebrarum are described. The lucent vacuoles in the foam cells were shown to be lamellar lipid structures in freeze etched preparations. No definite membrane of lipid vacuoles could be seen. The pattern of lamellar lipid in the foam cells differed from that in subcutaneous fat cells. Filaments in the cytoplasm of the foam cells were also observed and their possible function is discussed.
Zusammenfassung
Untersuchungen bei einem Patienten mit Xanthelasma palpebrarum nach Gefrierätzung des Gewebes werden mitgeteilt. Es zeigte sich, daß die offenen Vacuolen der Schaumzellen lamellare Lipidstrukturen aufwiesen, wenn das Gewebe nach dem Gefrierätzverfahren aufbereitet wurde. Die Lipidvacuolen wiesen keine erkennbaren Membranen auf. Die lamellaren Lipidstrukturen der Schaumzellen unterschieden sich weiterhin von den subcutanen Fettzellen.
Im Cytoplasma der Schaumzellen wurden Filamente beobachtet, deren mögliche Rolle erörtert wird.
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References
Breathnach, A. S.: An atlas of the ultrastructure of human skin, pp. 370–374. London: J. & A. Churchill 1971
Breathnach, A. S.: Freeze-cleavage replication of human skin. Read at International Congress of Dermatology, Venice, May 26, 1972
Buchheim, W.: Verteilung hochschmelzender Triglyceride in Fettkügelchen der Milch. Naturwissenschaften57, 41 (1970)
Fluck, D. J., Henson, A. F., Chapman, D.: The structure of dilute lecithin-water systems revealed by freeze-etching and electron microscopy. J. Ultrastruct. Res.29, 416–429 (1969)
Fujita, H., Asagami, C.: Electron microscopy of the embryonic differentiation of fat cells. Microscopie ElectroniqueIII, 535–536 (1970)
Fujita, H., Asagami, C., Murota, S., Murozumi, S.: Ultrastructural study of embryonic sebaceous cells, especially of their sebum droplet formation. Acta derm.-venereol. (Stockh.)52, 99–115 (1972)
Hasegawa, T., Ueda, K.: The ultrastructure of human subcutaneous fat cells. Jap. J. Derm., Ser. B81, 467–469 (1971)
Hasegawa, T., Takeuchi, M.: Heating element for freeze-etching. J. Electron Microscopy21, 151–152 (1972)
Hasegawa, T., So, Y.: Fine structure of Trichophyton rubrum after freeze etching. Read at International Congress of Dermatology, Venice, May 24, 1972
Hasegawa, T.: Freeze-etching observations of Candida albicans. Read at International Congress of Dermatology, Venice, May 24 1972
Hasegawa, T., Takeuchi, M., Uyeda, K.: Freeze etching observation of subcutaneous fat cells. Jap. J. clin. Electron Microscopy5, 235–236 (1972)
Hirone, T., Fukushiro, R.: Electron microscopic study on the formation of lipid droplets in xanthoma cells. Jap. J. clin. Electron Microscopy5, 233–234 (1972)
Imaeda, T.: Electron microscopic study of xanthoma cells. J. invest. Derm.34, 331–337 (1960)
Mühlethaler, K.: Studies on freeze-etching of cell membrane. Int. Rev. Cytol.31, 1–19 (1971)
Parker, F., Odland, G. F.: Electron microscopic similarities between experimental xanthomas and human eruptive xanthomas. J. invest. Derm.52, 136–147 (1969)
Reed, R., Rothwell, P. J.: Epidermal-dermal junction of normal human skin as seen in freeze-etched preparation. Brit. J. Derm.82, 470–486 (1970)
Sato, S.: Ultrastructure of experimental rabbit xanthoma. Jap. J. Derm., Ser. A82, 1061–1077 (1972)
Sheldon, H., Ferguson, C. C.: On the occurrence of cytoplasmic filaments in adipose cells. Microscopie ElectroniqueIII, 535–536 (1970)
Suzuki, H.: Electron microscopic findings of the xanthoma tuberosum. Jap. J. Derm., Ser. B79, 265–276 (1969)
Wolff, H. H., Braun-Falco, O.: Die Ultrastruktur des Xanthelasma palpebrarum. Arch. klin. exp. Derm.238, 308–322 (1970)
Yamamoto, K., Nakamura, R.: Fine structure of tuberous xanthoma. Jap. J. clin. Derm.23, 1064–1069 (1969)
Zemel, H., Deeken, J., Asel, N., Packer, J.: The ultrastructural features of normolipemic plane xanthoma. Arch. Path.89, 111–117 (1970)
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Hasegawa, T., Uyeda, K. Ultrastructure of Xanthelasma palpebrarum as revealed by freeze etching techniques. Arch. Derm. Forsch. 248, 347–354 (1974). https://doi.org/10.1007/BF00595553
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DOI: https://doi.org/10.1007/BF00595553