Summary
The way in which the epidermis migrates around the dermal aspect of fragments of skin in vitro has been investigated. Altogether 137 samples of adult human skin cultured for periods of 2–72 h have been examined. Migration was first detected at 22 h. The presence of serum in the medium promoted epidermal migration and this property was independent of any activity the serum might have had in maintaining tissue integrity. The epidermis of the migrating portion showed increased metabolic activity compared to the parent epidermis as evidenced by increased reactivity for the mitochondrial enzymes using histochemical methods. Migrant cells were shown electron microscopically to possess an increased number of ribosomes and polyribosomes. Intercellular contacts in the epibolic epidermis were only partially desmosomes. There were, in addition, villous structures that seemed to replace the usual desmosomal structures in places.
Zusammenfassung
Die Wanderung von Epidermiszellen entlang der Dermis wurde bei excidierten Hautstückchen in vitro untersucht. Insgesamt wurden 137 Hautexplantate von erwachsenen Probanden für die Dauer von 2–72 Std kultiviert. Die epitheliale Zellmigration begann nach 22 Std. Serum förderte die epidermale Zellmigration unabhängig von den kulturerhaltenden Eigenschaften des Serums. Histochemische Nachweisreaktionen für mitochondriale Enzyme zeigten, daß die auswandernde Epidermis eine höhere metabolische Aktivität aufwies als die Ursprungsepidermis. In den ausgewanderten Zellen war die Zahl elektronenmikroskopisch erkennbarer Ribosomen und Polyribosomen erhöht. Die intercelluläre Verbindung in der ausgewanderten Epidermis bestand nur teilweise aus Desmosomen, die von villösen Strukturen ersetzt zu sein schienen.
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Marks, R., Bhogal, B. & Dawber, R.P.R. The migratory property of epidermis in vitro. Arch. Derm. Forsch. 243, 209–220 (1972). https://doi.org/10.1007/BF00595497
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DOI: https://doi.org/10.1007/BF00595497