Summary
In normal hairless mouse epidermis the cellular respiration (QO2) on endogenous substrate in vitro is more sensitive to pH variations than the respiration with glucose in the medium.
During the first week after painting with the strong carcinogen 20-methylcholanthrene (MCA) the QO2 of hairless mouse epidermis is not more susceptible to pH variations than that of untreated epidermis. A transient broader pH optimum of the respiration to the acidic side after application of the solvent benzene or the irritant cantharidin in benzene is probably due to intracellular accumulation of lactic acid.
While the tetrazolium method shows a specific reaction pattern after MCA painting, the oxygen consumption mostly shows an unspecific pattern, reflecting altered cell population kinetics in the epidermis. After MCA treatment there is a discrepancy between oxygen consumption and formazan deposition until the 7th day. The oxygen consumption is decreased while the tetrazolium method gives increased or normal values. A possible explanation is that the mitochondrial membrane has an increased permeability for tetrazolium.
Zusammenfassung
Der O2-Verbrauch (QO2) der Epidermis haarloser Mäuse reagiert in vitro ohne Glucose im Inkubationsmedium empfindlicher auf pH-Schwankungen.
Nach einwöchiger Vorbehandlung der Epidermis mit Methylcholanthren (MCA) bestehen zwischen behandelter und unbehandelter Epidermis keine Unterschiede in der pH-Empfindlichkeit des QO2.
Eine vorübergehende Verbreiterung des pH-Optimums zur sauren Seite nach Benzol oder Cantharidin in Benzol hat eine intracelluläre Anhäufung von Milchsäure als wahrscheinliche Ursache.
Die Tetrazoliummethode zeigt ein spezifisches Reaktionsverhalten nach MCA-Vorbehandlung. Dagegen reagiert der O2-Verbrauch uncharakteristisch als Ausdruck einer veränderten epidermalen Zellkinetik. Nach MCA-Pinselung kommt es bis zum 7. Tag zu einer Erniedrigung des O2-Verbrauches, während die Tetrazoliummethode normale bis erhöhte Werte aufweist. Die Resultate lassen sich mit einer erhöhten Permeabilität der Mitochondrienmembran für Tetrazolium erklären.
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Laerum, O.D., Iversen, O.H. pH-dependence of epidermal respiration under normal conditions and in chemically induced hyperplasia. Arch. Derm. Forsch. 246, 92–102 (1973). https://doi.org/10.1007/BF00595491
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DOI: https://doi.org/10.1007/BF00595491