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Blood group substance a prepared from human seminal plasma

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Summary

The blood group A substance occurring in the human seminal plasma has been isolated and purified to a certain degree by phenol/saline extraction. Immunological and biochemical investigations have shown that it is a glycoprotein with the electrophoretic mobility of aβ-globulin containing 7.38% N-acetylneuraminic acid.

Immunization of a rabbit with pooled human seminal plasma proteins yielded an antiserum of high quality containing “incomplete” agglutinating antibodies against human red blood cells but no precipitating antibodies against the blood group A substance.

Zusammenfassung

Die Blutgruppensubstanz A des menschlichen Spermaplasma wurde durch Phenol-Kochsalz-Extraktion isoliert und weitgehend gereinigt. Immunologische und biochemische Analysen ergaben, daß es sich um ein Glykoprotein mit der elektrophoretischen Beweglichkeit einesβ-Globulins handelt, das 7,38% N-acetyl-neuraminsäure enthält.

Die Immunisierung eines Kaninchens mit gepooltem menschlichem Spermaplasma ergab ein sehr gutes Antiserum, das „inkomplette“ agglutinierende Antikörper gegen menschliche Erythrocyten enthielt, aber keine präcipitierenden Antikörper gegen die Blutgruppensubstanz A.

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References

  1. Baldo, B. A., Boettcher, B.: Examination of the semen and erythrocytes of seven species for immunological cross-reaction. J. Reprod. Fertil.24, 391–401 (1971).

    Google Scholar 

  2. Böhm, P.: Die Neuraminsäure (Biochemie, Biologie und Klinik). Dtsch. Z. Verdau.- u. Stoffwechselkr.19, 65–87 (1959).

    Google Scholar 

  3. Herrmann, W. P.: Immunologische Charakterisierung menschlicher Spermaplasmaproteine. Andrologie1, 11–18 (1969).

    Google Scholar 

  4. Herrmann, W. P., Uhlenbruck, G.: Studies on a seminal plasma protein with blood group A Specificity. Arch. Derm. Forsch.245, 170–176 (1972).

    Google Scholar 

  5. Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the folin phenol reagent. J. biol. Chem.193, 265–275 (1961).

    Google Scholar 

  6. Ouchterlony, Ö.: Antigen-antibody reactions in gels. Ark. Kemi,26B, 1–9 (1948).

    Google Scholar 

  7. Prokop, O., Uhlenbruck, G.: Lehrbuch der menschlichen Blut- und Serumgruppen. Leipzig: VEB Thieme 1966.

    Google Scholar 

  8. Prokop, O., Uhlenbruck, G., Köhler, A.: A new source of antibody-like substances having anti-blood group specificity. Vox Sang. (Basel)14, 321–333 (1968).

    Google Scholar 

  9. Prokop, O., Uhlenbruck, G., Schnitzler, S.: Vergleichende Untersuchungen mit Eiweißdrüsenextrakten von Helix pomatia und Helix hortensis Bibl. haemat. (Basel)27, 108–114 (1967).

    Google Scholar 

  10. Scheidegger, J. J.: Une micro-méthode de l'immuno-électrophorèse. J. Int. Arch. Allergy7, 103–110 (1955).

    Google Scholar 

  11. Uhlenbruck, G., Dahr, W.: Studies on lectins with a broad agglutination spectrum. XII. N-acetyl-D-galactosamine specific lectins from the seeds of Soja hispida, Bauhinia purpurea, Iberis amara, Moluccella laevis and Vicia graminea Vox Sang. (Basel)21, 338–351 (1971).

    Google Scholar 

  12. Uhlenbruck, G., Herrmann, W. P.: Agglutination of normal, coated and enzymetreated human spermatozoa with heterophile agglutinins. Vox Sang. (Basel)23, 444–451 (1972).

    Google Scholar 

  13. Uhlenbruck, G., Sprenger, I., Pardoe, G. I.: Über Lektine mit einem breiten Agglutinationsspektrum. VIII. Serologische Eigenschaften der Blutgruppensubstanz A aus Pepton. Immunforsch.140, 496–504 (1970).

    Google Scholar 

  14. Watkins, W. M.: Blood group specific substances. In: Glycoproteins, Vol. 5, pp. 462–515. Ed. A. Gottschalk. Amsterdam-London-New York: Elsevier Publ. Comp. 1966.

    Google Scholar 

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Authors and Affiliations

  1. Department of Dermatology, University of Cologne, FRG

    G. Uhlenbruck, Gisela Steinhausen & W. P. Herrmann

  2. Department of Medicine, University of Cologne, FRG

    G. Uhlenbruck, Gisela Steinhausen & W. P. Herrmann

Authors
  1. G. Uhlenbruck
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  2. Gisela Steinhausen
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  3. W. P. Herrmann
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Additional information

This investigation was in part supported by the Deutsche Forschungsgemeinschaft.

With technical assistance of I. Weyland.

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Uhlenbruck, G., Steinhausen, G. & Herrmann, W.P. Blood group substance a prepared from human seminal plasma. Arch. Derm. Forsch. 246, 47–54 (1973). https://doi.org/10.1007/BF00595486

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  • DOI: https://doi.org/10.1007/BF00595486

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