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Archiv für dermatologische Forschung

, Volume 246, Issue 4, pp 383–400 | Cite as

Veränderungen der Ultrastruktur menschlicher Geschwülste durch Übertragung auf Labortiere

I. Melanomalignom (4 Fälle)
  • Rajmund Dvořák
  • Michael Jänner
  • Uta Eckhardt
Article

Zusammenfassung

Melanomalignome von 4 Patienten wurden auf xenogene Wirte übertragen. Ratten und Meerschweinchen bekamen kleine Tumorfragmente in die vordere Augenkammer implantiert, Hamstern wurden Geschwülste in die Backentaschen übertragen. Die Gewebsinkompatibilität der Tumorempfänger wurde mit Cyclophosphamid und Corticosteroid gehemmt. Die einzelnen Tumorwirte wurden zwischen dem 10. und 46. Tag nach der Tumorübertragung getötet und untersucht.

Im Cytoplasma der übertragenen Tumorzellen wie auch in manchen, im Tumorbereich vorhandenen Wirtsleukocyten wurden zunächst beträchtliche Anhäufungen der ribosomenähnlichen Partikeln gesehen. Später waren stellenweise in den Tumorzellen virusähnliche Körperchen und Partikeln vom Typ der Mykoplasma-Elementarkörperchen vorhanden. Manche lysosomenartige Gebilde sahen wie intracellulär etablierte Mykoplasmen aus. Besonders auffällig schienen bestimmte längliche membranumschlossene Gebilde, die durch eine dicke, quergestreifte Faser in ihrem Zentrum und Ansammlungen der ribosomenartigen Granula an ihren Enden gekennzeichnet waren.

Die Möglichkeit einer Symbiose von Tumorzellen und Mykoplasmen und einer vermuteten larvierten intracellulären Mykoplasmenreproduktion wird diskutiert.

Ultrastructural changes of human tumours after transfer into laboratory animals

I. Malignant Melanoma (4 Cases)

Summary

Malignant melanomas from 4 Patients were transferred into xenogeneic hosts. Rats and guinea-pigs received small tumour fragments, implanted in their anterior eye chamber. The tumours were also implanted in the cheek pouches of hamsters. The tissue incompatibility of tumour receivers was suppressed using cyclophosphamide and corticosteroid. The tumour hosts were killed and examined 10–46 days after tumour transfer.

At first there were remarkable accumulations of ribosome-like particles seen in the cytoplasm of the transferred tumour cells and in some host leukocytes found in the tumour area. Later viruslike bodies and particles of the mycoplasma elementary body type were present in the tumour cells. Various lysosome-like formations appeared similar to intracellular mycoplasmas. Elongated membrane-bound structures, characterized by a stout, cross-striated fibre in their centre and collections of ribosome-like granula at their ends, were particularly conspicuous.

The possibility of a symbiosis between the tumour cells and mycoplasmas, and of a reproduction of mycoplasmas in human tumour cells is discussed.

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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • Rajmund Dvořák
    • 1
  • Michael Jänner
    • 1
  • Uta Eckhardt
    • 1
  1. 1.Hautklinik der Universität HamburgBRD

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