Archiv für dermatologische Forschung

, Volume 248, Issue 1, pp 89–98 | Cite as

Autoradiographische Untersuchungen über den Einfluß von Cyproteronacetat auf die epidermale Proliferation der männlichen Ratte

  • H. Schell
  • G. H. Schmid
Article

Zusammenfassung

An 12 erwachsenen, männlichen Wistarratten wurde der Einfluß von Cyproteronacetat (tägliche Gabe von 10 mg/Tier) auf die DNS-Synthese im interfollikulären Epidermisbereich der Rückenhaut, im stratum basale der Wangenschleimhaut und im Samenblasenepithel untersucht. Parallel dazu wurde die Proliferation dieser 3 Epithelien nach 90 tägiger, operativer Kastration geprüft. Die DNS-Synthese wurde autoradiographisch nach Gabe von3H-Thymidin (500 µC/Tier) bestimmt.

Es zeigte sich, daß unter den gewählten Versuchsbedingungen der3H-Index in der Epidermis und in der Wangenschleimhaut nicht beeinflußt wurde; im Gegensatz dazu stand der bereits nach 24 Std nachweisbare, starke Abfall des3H-Indexes im Samenblasenepithel. Die Relevanz dieser Befunde für die epidermale Proliferation wird diskutiert.

Autoradiographic investigations on epidermal proliferation in male rats after Cyproteronacetate administration

Summary

In 12 adult male rats we studied the influence of Cyproteronacetate (10 mg/day) on DNA-synthesis in the interfollicular epidermis of the back, in the basal cell layer of the buccal mucosa, and in the epithelium of the vesicular glands. In addition, 2 other rats were castrated by orchidectomy and investigated in the same manner. After the application of3H-thymidine (500 µC/animal) autoradiographic studies on DNA-synthesis were performed.

The labelling index in the epidermis and in the buccal epithelium was not altered—neither by hormonal nor by operative castration, while it was extremely depressed in the vesicular glands.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literatur

  1. Allen, J. M.: Influence of hormones on cell division. II. Testosteroneproprionate. Anat. Rec.128, 515 (1957)Google Scholar
  2. Bardin, C. W., Peterson, R. E.: Studies of androgen production by the rat: testosterone and androstendione content of blood. Endocrinology80, 38–44 (1967)Google Scholar
  3. Cameron, E. H. D., Baillie, A. H., Grant, J. K., Milne, J. A., Thomson, J.: Transformation in vitro of (7α-3H)-dehydroepiandrosterone to (3H)-testosterone by skin from men. J. Endocr.35, XIX-XX (1966)Google Scholar
  4. Darcis, L.: Influence de la testosterone sur l'activité mitotique du vagin, du rectum et de la peau chez la ratte. Ann. Endocr. (Paris)23, 545–555 (1962)Google Scholar
  5. Dorfman, R. I., Shipley, R. A.: Androgens. New York: Wiley 1965. Zit. nach P. Hall: Endocrinology in the testis, pp. 1–71. In: The Testis. Eds.. A. D. Johnson, W. R. Gomes, and N. L. Vandenmark. New York: Academic Press 1970Google Scholar
  6. Eartley, H., Grad, B., Leblond, C. P.: The antagonistic relationship between testosterone and thyroxine in maintaining the epidermis of the male rat. Endocrinology49, 677–686 (1951)Google Scholar
  7. Ebling, F. J.: The action of testosterone on the sebaceous glands and epidermis in castrated and hypophysectomized male rats. J. Endocr.15, 297–306 (1957)Google Scholar
  8. Ebling, F. J.: The effects of steroid hormones on the skin of experimental animals. Symp. Dtsch. Ges. Endokrin., vol. 17, pp. 19–32. Berlin-Heidelberg-New York: Springer 1971Google Scholar
  9. Epifanova, O. I.: Effects of hormones on the cell cycle, pp. 145–226. Ed.: R. Baserga. New York: Marcell Dekker, Inc. 1971Google Scholar
  10. Fang, S., Liao, Sh.: Androgen receptors. Steroid- and tissue-specific retention of a 17β-hydroxy-5α-androstan-3-one-protein complex by the cell nuclei of ventral prostate. J. biol. Chem.246, 16–24 (1971)Google Scholar
  11. Faredin, I., Webb, J. L., Julesz, M.: The in vitro metabolism of dehydroepiandrosterone in human skin. Acad. Sci. Acta med. hung.23, 169–180 (1967)Google Scholar
  12. Gomez, E. C., Hsia, S. L.: Studies on cutaneous metabolism of testosterone-4-14C. Fed. Proc.25, 282 (1966)Google Scholar
  13. Gomez, E. C., Hsia, S. L.: In vitro metabolism of testosterone-4-14C and Δ-4-androstene-3, 17-dione-4-14C in human skin. Biochemistry7, 24–32 (1968)Google Scholar
  14. Henry, J. L., Meyer, J., Weinmann, J. P., Schour, I.: Pattern of mitotic activity in oral epithelium of rabbits. Arch. Path.54, 281–297 (1952)Google Scholar
  15. Hsia, S. L.: Steroid metabolism in human skin, pp. 69–88. In: Modern trends in dermatology. Ed.: P. Borrie. London: Butterworths 1971Google Scholar
  16. Knorr, D.: Testosteron und Pubertät bei Knaben. 16. Symp. Dtsch. Ges. Endokrin. pp. 151–165. Berlin-Heidelberg-New York: Springer 1970Google Scholar
  17. Mann, T. A.: The biochemistry of semen and of the male reproductive tract. London: Methuen and Co. Ltd., New York: John Wiley and Sons Inc. 1964Google Scholar
  18. Montagna, W., Kenyon, P., Hamilton, J. B.: Mitotic activity in the epidermis of the rabbit stimulated with local applications of testosterone propionate. J. exp. Zool.110, 379–395 (1949)Google Scholar
  19. Mosebach, K. O.: Verteilung, Bindungen und Metabolite von Testosteronen im Säugetierorganismus, pp. 17–31. In: Das Testosteron — Die Struma. 13. Symp. Dtsch. Ges. Endokrin. Berlin-Heidelberg-New York: Springer 1968Google Scholar
  20. Neumann, F., von Berswordt-Wallrabe, R., Elger, W., Steinbeck, H.: Hormonhemmer — Untersuchungen mit Testosteron-Antagonisten. 18. Coll. Ges. Phys. Chem., Mosbach 1967. In: Wirkungsmechanismus der Hormone, pp. 218–254. Ed.: P. Karlson. Berlin-Heidelberg-New York: Springer 1967Google Scholar
  21. Neumann, F., Elger, W., Steinbeck, H., von Berswordt-Wallrabe, R.: Antiandrogene, pp. 78–101. 13. Symp. Dtsch. Ges. Endokrin., Würzburg 1967. In: Das Testosteron — Die Struma. Ed. E. Klein. Berlin-Heidelberg-New York: Springer 1968Google Scholar
  22. Ofner, P.: Recent developments in the study of hormone effects and metabolism in prostate tissue. 17. Symp. Dtsch. Ges. Endokrin., pp. 147–156. Berlin-Heidelberg-New York: Springer 1971Google Scholar
  23. Overbeck, G. A.: Anabole Steroide. Berlin-Heidelberg-New York: Springer 1966Google Scholar
  24. Papa, C. M., Kligman, A. M.: The effect of topical steroids on the aged human axilla, pp. 177. Advances in Biology of Skin, Vol. 6. Ed. W. Montagna. Oxford: Pergamon Press 1965Google Scholar
  25. Reaven, E. P., Cox, A. J.: Behavior of adult human skin in organ culture. J. invest. Derm.50, 118–128 (1968)Google Scholar
  26. Sandler, R., Hall, P. F.: The influence of age upon the response of rat testis to ICSH in vitro. Biochim. biophys. Acta (Amst.)164, 445–447 (1968)Google Scholar
  27. Schaaf, F.: Probleme dermatologischer Grundlagenforschung, pp. 111. Heidelberg: Dr. A. Hüthig 1969Google Scholar
  28. Schmid, G. H., Arnold, V., Hornstein, O. P.: Das Wachstumsverhalten des Samenblasenepithels der erwachsenen weißen Ratte nach Kastration. Virchows Arch., Abt. B, Zellpath.8, 361–364 (1971)Google Scholar
  29. Schmid, G. H., Rathgeber, D.: Einfluß des Narkoseverfahrens auf die postoperative Samenblasenproliferation der erwachsenen weißen Ratte. Virchows Arch., Abt. B Zellpath.11, 274–278 (1972)Google Scholar
  30. Steigleder, G. K., Buchwald, W.: Experimentelle Untersuchungen zum Verhalten der verbreiterten Rattenepidermis. Hautarzt8, 505–509 (1957)Google Scholar
  31. Steigleder, G. K., Schultis, K.: Experimentelle Untersuchungen zur Epidermisverbreiterung. Arch. klin. exp. Derm.202, 567–576 (1956)Google Scholar
  32. Steinbeck, H., Mehring, M., Neumann, F.: Comparison of the effects of cyproterone, cyproterone acetate and oestradiol on testicular function, accessory sexual glands and fertility in a long-term study on rats. J. Reprod. Fertil.26, 65–76 (1971)Google Scholar
  33. Stern, J. M., Eisenfeld, A. J.: Androgen accumulation and binding to macromolecules in seminal vesicles: inhibition by cyproterone. Science166, 233–235 (1969)Google Scholar
  34. Studer, A., Frey, J. R.: Wirkung von Cortison auf die ruhende und die mit Vitamin A oder Testosteronpropionat zur Proliferation gebrachte Epidermis der Ratte. Dermatologica (Basel)104, 1–18 (1952)Google Scholar
  35. Tausk, M.: Pharmakologie der Hormone. Stuttgart: Thieme 1970Google Scholar
  36. Tuohimaa, P., Niemi, M.: The effect of testosterone on cell renewal and mitotic cycles in sex accessory glands of castrated mice. Acta endocr. (Kbh.)58, 696–704 (1968)Google Scholar
  37. Vida, I. A.: Androgens and anabolic agents: chemistry and pharmacology, p. 5. New York: Academic Press 1969Google Scholar
  38. Walsh, P. C., Korenman, S. G.: Mechanism of adrogenic action: effect of specific intracellular inhibitors. J. Urol. (Baltimore)105, 850–857 (1971)Google Scholar
  39. Wilson, J. D.: Testosterone metabolism in skin. 17. Symp. Dtsch. Ges. Endokrin., pp. 11–18. Berlin-Heidelberg-New York: Springer 1971Google Scholar
  40. Wilson, J. D., Walker, J. D.: The conversion of testosterone to 5 α-androstan-17β-ol-3-one (dihydrotestosterone) by skin slices of man. J. clin. Invest.48, 371–379 (1969)Google Scholar
  41. Winkler, K.: Hormonbehandlung in der Dermatologie. Berlin: W. de Gruyter u. Co. 1969Google Scholar
  42. Wotiz, H. H., Mescon, H., Doppel, H., Lemon, H. M.: The in vitro metabolism of testosterone by human skin. J. invest. Derm.26, 113–120 (1956)Google Scholar
  43. Zackheim, H. S.: Effect of prolonged administration of testosterone on the epidermis of female rats. J. invest. Derm.50, 401–404 (1968)Google Scholar

Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • H. Schell
    • 1
  • G. H. Schmid
    • 1
  1. 1.Dermatologische Universitätsklinik ErlangenBRD

Personalised recommendations