Skip to main content
SpringerLink
Log in

Anticoagulants for avian and reptilian blood: Heparin and EDTA

  • Instruments and Techniques
  • Published:
Pflügers Archiv Aims and scope Submit manuscript

Summary

The effectiveness of EDTA (dipotassium and disodium salts) and heparin as anticoagulants for the blood of puffadders and pigeons has been investigated. In the case of the snakes all three substances produced haemolysis after variable time intervals, and increases in cell volume were observed. In the case of the pigcons, samples stored at 4° C showed lower haematocrit values and haemolysed sooner than samples stored at room temperature.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Blaxhall, P. C.: The haematological assessment of health of freshwater fish. J Fish Biol.4, 593–605 (1972)

    Google Scholar 

  • Cohen, F. L.: Anticoagulation, centrifugation time and sample replicate number in the microhaematocrit, using Cr51. Poultry Sci.47, 219–233 (1966)

    Google Scholar 

  • Dessauer, H. D.: Blood chemistry of reptiles: Physiological and evolutionary aspects. In: Biology of the reptilia, vol. IV (C. Gans, ed.), pp. 1–72. London-New York: Academic Press 1970

    Google Scholar 

  • Hamre, C. J., McHenry, J. T.: Methods of obtaining blood of fowl for complete blood examination. Poultry Sci.21, 30–34 (1942)

    Google Scholar 

  • Hattingh, J.: Heparin and ethylenediamine tetra-acetate as anticoagulants for fish blood. Pflügers Arch.355, 347–352 (1975)

    Google Scholar 

  • Lyman, R. A.: The anti-haemolytic function of calcium in the blood of the snapping turtle,Chelydra sepentina. J. cell. comp. Physiol.25, 65–73 (1945)

    Google Scholar 

  • McMahan, J. A., Hamer, A. H.: Haematology of the sidewinder. Comp. Biochem. Physiol.51A, 53–58 (1975)

    Google Scholar 

  • Morten, J. H., Bagby, R. M.: Effects of fasting on the blood chemistry of the rattlesnake,Crotalus atrox. Comp. Biochem. Physiol.44, 813–820 (1973)

    Google Scholar 

  • Pienaar, U de V.: Haematology of some South African reptiles. Johannesburg: Witwatersrand University Press, 1962

    Google Scholar 

  • De Villiers, O. T.: The blood of the ostrich. Onderstepoort J. vet. Sci. Anim. Ind.2, 419–503 (1938)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Physiology, Medical School, University of the Witwatersrand, 2001, Johannesburg, South Africa

    J. Hattingh

  2. Department of Zoology, Rand Afrikaans University, 2001, Johannesburg, South Africa

    E. M. Smith

Authors
  1. J. Hattingh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. M. Smith
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hattingh, J., Smith, E.M. Anticoagulants for avian and reptilian blood: Heparin and EDTA. Pflugers Arch. 363, 267–269 (1976). https://doi.org/10.1007/BF00594613

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00594613

Key words

Search

Navigation