Summary
The incident-light, high-frequency microcinematography of the kidney surface of rats is described as a new method for investigating renal microcirculation. The welling point area served as the anatomical reference point for microcirculatory measurements. In the first section of vessel distal to the welling point the average flow velocity was 2.32±0.24 mm/sec; the diameter was 15.91±0.92 microns. Flow rate in the efferent arteriole, calculated from the flow rate in the first vessel section distal to the welling point and the degree of ramification, was 103 nl/min. The results closely agree with total renal blood flow that was determined simultaneously.
As an example for measurements taken under different conditions, a model for experimental acute renal failure was chosen. In kidneys subjected to a 60 min period of ischemia three days prior to use, the peritubular blood flow at the kidney surface was decreased only by 1/4; the difference in the means was insignificant. Therefore, a lasting cortical ischemia cannot be the only cause for the persistent oliguria in experimental acute renal failure.
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Steinhausen, M., Eisenbach, G.M. & Böttcher, W. High-frequency microcinematographic measurements on peritubular blood flow under control conditions and after temporary ischemia of rat kidneys. Pflugers Arch. 339, 273–288 (1973). https://doi.org/10.1007/BF00594163
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DOI: https://doi.org/10.1007/BF00594163