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Cryosopic determination of renal tissue osmolality by an ultramicro method

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Summary

A micromethod for the determination of tissue osmolality is described. In essence it consists of ultracentrifugation of tissue and cryoscopic analysis of osmolarity of the supernatant in a nanoliter osmometer. The method was applied to renal cortical and medullary tissues in rats with and without Diabetes insipidus, and in dogs.

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Authors and Affiliations

  1. Department of Physiology, Dartmouth Medical School, Hanover, New Hampshire, USA

    W. M. Kettyle, M. Horster (Recipient of Fellowship for Foreign Scientists, National Kidney Foundation, New York), K. Thurau (Prof. of Physiology, University of Munich and Visiting Gilman Professor of Physiology, Dartmouth Medical School) & H. Valtin (Recipient of U.S. Public Health Service Research Career Program Award 6-K3-GM 21, 786 from the National Institute of General Medical Sciences)

  2. Physiologisches Institut der Universität, Pettenkoferstraße 12, D-8000, München 15

    K. Thurau (Prof. of Physiology, University of Munich and Visiting Gilman Professor of Physiology, Dartmouth Medical School)

Authors
  1. W. M. Kettyle
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  2. M. Horster
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  3. K. Thurau
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  4. H. Valtin
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Additional information

Supported by U.S. Public Health Service Research Grant AM-08469-GM from the National Institute of Arthritis and Metabolic Diseases.

Predoctoral Trainee in Physiology, supported by National Heart Institute Training Grant HE-5322.

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Kettyle, W.M., Horster, M., Thurau, K. et al. Cryosopic determination of renal tissue osmolality by an ultramicro method. Pflugers Arch. 321, 83–89 (1970). https://doi.org/10.1007/BF00594124

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  • DOI: https://doi.org/10.1007/BF00594124

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