Zusammenfassung
Durch Verabreichung von Methylandrostenediol entstanden hyaline Tröpfchen und eiweißhaltige Vacuolen in Nebennierenrindenzellen bei weiblichen weißen Ratten. Die cytoplasmatischen Ablagerungen zeigten keine positive alkalische Phosphatasereaktion bei entsprechenden Färbungen, jedoch eine positive Farbreaktion bei saurer Phosphatase und unspezifischer Esterase. Die dichteren und regelmäßigeren Tröpfchen wiesen eine gleichmäßigere und kräftigere Fermentaktivität auf. Dagegen enthielten die vacuolären Ablagerungen wasserhaltige Substanzen. Die als frischer abgefaßte Ablagerungen war enauf saure Hydrolasen schwach positiv. Mit immunohistologischen Methoden konnte man nachweisen, daß sie homologes Plasmaeiweiß enthielten. In den meisten Vacuolen fanden sich ausschließlich Albumine, einige enthielten jedoch auch Globuline und Fibrin in unterschiedlicher Menge Die gleichzeitige Verwendung von verschiedenen, mit FITC und LRB 200 markierten Antikörpern erbrachte den Nachweis, daß die Tröpfchen aus einer Plasma-Eiweiß-Mischung bestanden. Durch die immunohistologische Methode wurde deutlich gemacht, daß die Tröpfchen nicht homogen sind. Man darf annehmen, daß die intracellulären „hyalinen“ Ablagerungen eine gewöhnliche Reaktion der Nebennierenrindenzellen darstellt, die mit den Eiweiß-resorption-Tröpfchen, Phagosomen und deren weiteren Entwicklungsstadien wie sie in anderen Organen vorkommen, vergleichbar sind. So werden diese Gebilde mit dem pinocytär-lysosomalen System der Nebennierenrindenzelle in Bezug gebracht.
Summary
Hyaline droplets and protein-containing vacuoles were produced in adrenocortical cells of female albino rats by methylandrostenediol administration. The lesions were shown to be invariably negative with the method for alkaline phosphatase, but they showed a varying degree of positive staining with the reactions for acid phosphatase and nonspecific esterases. The denser and more regular “droplets” tended to exhibit a more regular and stronger enzymatic activity, whereas the lesions of the “vacuole” type containing a less dense, presumably watery material; those regarded as “younger” were only faintly, if ever, positive with the methods for acid hydrolases.
With the immunohistochemical method the authors were able to demonstrate the presence of homologous plasma proteins within the lesions. Most of the droplets and vacuoles were shown to contain albumin, and some of them could be demonstrated to contain also globulin and fibrin in varying amounts. The simultaneous use of different antibodies labelled with contrasting fluorescent dyes as fluorescein isothiocyanate and lissamine rhodamine B 200, disclosed that most droplets were composed of a mixture of plasma proteins. With that method, it was possible to visualise inhomogeneities in the composition of droplets which largely corresponded to those described previously in preparations stained with conventional histological techniques as well as with some histochemical methods.
The authors conclude that this type of intracellular “hyaline” deposit represents a rather common reaction of adrenocortical cells and that it is comparable to the protein absorption droplets, phagosomes and their subsequent developmental stages known in other organs, thus being related to the pinocytotic-lysosomal system of the adrenocortical cell.
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Motlík, K., Krawczyński, K. & Nowosławski, A. Experimental hyaline droplets in the rat adrenal cortex. Virchows Arch. Abt. A Path. Anat. 344, 331–345 (1968). https://doi.org/10.1007/BF00593542
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DOI: https://doi.org/10.1007/BF00593542