Lasers in Medical Science

, Volume 13, Issue 1, pp 14–21 | Cite as

Diagnostic imaging using rare-earth chelates

  • D. S. Hubbard
  • M. P. Houlne
  • G. Kiefer
  • H. F. Janssen
  • C. Hacker
  • D. J. Bornhop
Original Articles

Abstract

A new class of polyazamacrocyclic chelates of terbium is studied that have rich spectroscopic properties, tissue selectivity [1], millisecond fluorescence lifetimes, sharply spiked emission spectra (<15 nm FWHM), large Stokes shifts (>280 nm), good water solubility [1] and high fluorescence quantum yields (∼0.6 for PCTMB) [1]. We report our in vitro and ex vivo spectroscopic evaluation of these chelates. Additionally, results from cellular binding specificity studies using Sprague-Dawley rats with UMR 108 osteosarcomas are presented. Finally, endoscopic imaging of dosed tissue demonstrates the potential to use the Tb(III) chelates as contrast enhancement markers.

Keywords

Fluorescence Lanthanide chelate Probes Tissue selectivity 

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Copyright information

© Springer-Verlag 1998

Authors and Affiliations

  • D. S. Hubbard
    • 1
  • M. P. Houlne
    • 1
  • G. Kiefer
    • 2
  • H. F. Janssen
    • 3
  • C. Hacker
    • 3
  • D. J. Bornhop
    • 1
  1. 1.Department of Chemistry and BiochemistryTexas Tech UniversityLubbockUSA
  2. 2.Designed Chemicals R&D, Dow Chemical CompanyFreeport
  3. 3.Department of OrthopedicsTexas Tech University Health Sciences CenterLubbockUSA

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