Pflügers Archiv

, Volume 378, Issue 3, pp 243–249 | Cite as

Effect of external potassium on the coupled sodium: Potassium transport ratio of axons

  • Edward M. Lieberman
Excitable Tissues and Central Nervous Physiology

Abstract

  1. 1.

    Resting membrane potential and the current-voltage relation were measured in crayfish giant axons bathed in various potassium solutions with and without ouabain.

     
  2. 2.

    Ouabain caused a depolarization of the membrane at each [K] o used but did not affect membrane resistance.

     
  3. 3.

    The ouabain-sensitive transport current was least (3 μA/cm2) in 0 mM [K] o and greatest (7 μA/cm2) in 16.2 and 21.6 mM [K] o .

     
  4. 4.

    The assumption was made and some indirect evidence presented that axons equilibrated in various potassium solutions maintain constant internal sodium and potassium concentrations for up to 3 h.

     
  5. 5.

    On the basis of this assumption, the apparent ratio of coupled Na∶K transport was calculated. It was found to be least (−1.3/l) in 0 mM [K] o and to approach infinity in 16.2 and 21.6 mM [K] o .

     
  6. 6.

    The data indicate that the apparent variability of the Na∶K exchange ratio likely represents an intrinsic property of the exchange mechanism and is less likely to be explained by a fixed-ratio coupled Na∶K transport operating in parallel with electro-neutral Na∶Na or K∶K exchange.

     

Key words

Potassium Coupled Na∶K transport Axon Procambarus clarkii 

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Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Edward M. Lieberman
    • 1
  1. 1.Department of Physiology, School of MedicineEast Carolina UniversityGreenvilleU.S.A.

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