Summary
Fibres of frogs sartorius were extracted with glycerol and stored for up to 2 months at −16°C without losing their contractility, while the calcium-sensitivity was partly lost during longer storage. Freshly glycerinated (fully calcium-sensitive) fibres were suspended in ATP-salt-solution with 10−7–10−6 M Ca++ and fibres with partially lost calcium-sensitivity (older than 2 months) were suspended in calcium-free ATP-salt-solution (10−8 M Ca++).
Quick stretch by 0.1–1% of the fibre length caused an immediate tension rise followed by a small tension fall and a secondary delayed tension rise. The maximum tension, but not its kinetics decreased with decreasing actin-myosin-overlap (range 2.2–3 μm). After quick release the immediate tension fall was often followed by a small tension recovery passing into a delayed tension fall. Calcium was not essential for the delayed tension production if the fibres were aged or suspended at pH 8.
During sinusoidal length changes (0.1–1%L 0; 4–45 c/s) the sinusoidal tension changes lagged behind, so that the fibres produced positive work, which was maximum at the frequency of 20 c/s.
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Heinl, P. Mechanische Aktivierung und Deaktivierung der isolierten contractilen Struktur des Froschsartorius durch rechteckförmige und sinusförmige Längenänderungen. Pflugers Arch. 333, 213–226 (1972). https://doi.org/10.1007/BF00592684
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DOI: https://doi.org/10.1007/BF00592684