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Pflügers Archiv

, Volume 346, Issue 1, pp 39–48 | Cite as

Induction of Na K ATPase in the proximal and distal convolution of the rat nephron after uninephrectomy

  • Udo Schmidt
  • Ulrich C. Dubach
  • Barbara Funk
  • Kaija Paris
Article

Summary

Using refinements of quantitative histochemistry, i.e. oil-well technique and enzymaticP i analysis combined with an enzymatic cycling system, the activity of Na K ATPase (E.C. 3.6.1.3), an enzyme which is integrated in the transmembrane cation transport, was measured in single dissected segments of the proximal and distal convolution from the superficial nephron of the rat kidney following uninephrectomy. In the distal convolution an acute rise in Na K ATPase activity was apparent already during the first day after uninephrectomy, whereas in the proximal convolution the first significant increase occurred seven days after surgery. These data indicate a rapid adaptation of Na K ATPase which is limited to the distal tubule. The difference between the proximal and distal tubule confirms the functional correlations to uninephrectomy with more reabsorptive work in the distal tubule per unit length. Na K ATPase seems to have a special functional meaning for the distal tubule in regulating cation transport.

Key words

Na K ATPase Proximal Tubule Distal Tubule Rat Kidney Uninephrectomy Quantitative Histochemistry 

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Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • Udo Schmidt
    • 1
    • 2
  • Ulrich C. Dubach
    • 1
    • 2
  • Barbara Funk
    • 1
    • 2
  • Kaija Paris
    • 1
    • 2
  1. 1.Enzymlaboratorium der Medizinischen PoliklinikDep. für Innere Medizin der Universität BaselSchweiz
  2. 2.Pathologisches Institut der Universität TübingenGermany

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