Pflügers Archiv

, Volume 324, Issue 4, pp 328–343 | Cite as

Reexamination of the split oil droplet method as applied to kidney tubules

  • A. Z. Györy


The technique and evaluation of the split oil drop method of Gertz was reexamined in rats with a view to defining the correct dimension in which the results should be expressed so that the point of reference for reabsorptive capacity thus obtained be uninfluenced by the geometry of the tubule.

  1. 1.

    It was found that one tubular diameter had to be added to each length between the tops of the approaching menisci to produce a truly linear relationship between these lengths and time. This correction improved the coefficient of variation (reproducibility) of the reabsorptive half time (t1/2) and eliminated the correlation between the length of the split column andt1/2.

  2. 2.

    Furthermore a significant correlation was found between internal tubular radius and correctedt1/2's in a group of tubules with naturally varying diameters, indicating constancy of reabsorptive capacity per unit surface area.

  3. 3.

    The reproducibility of reabsorptive capacity per unit surface area,J v (a) thus obtained (coefficient of variation of 17%,n=44) was found to compare very favourably with other micropuncture techniques measuring the same parameter. The reproducibility of this parameter when expressed per unit tubular length,J v (l) was 23% (n=44). The accuracy of the method as measured by duplicate determinations was at 10% even better (n=38). From these considerations it was concluded that reabsorptive capacity of tubules should be expressed per unit area when comparisons between different tubules are to be made, and per unit length when comparisons of two or more determinations in the same tubule are to be compared.

  4. 4.

    The small difference inJ v found between measurements made with the Gertz method and microperfusion methods using TF/P inulin ratios was not due to the oil used nor was it due to a possible mechanical damage by the relatively large size of the double barrelled glass capillary usually employed in the Gertz method.



Renal Micropuncture Renal Proximal Tubular Sodium Reabsorption 


Mikropunktion Niere Proximale Tubuli Natrium Reabsorption 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Bank, N., Koch, K. M., Aynedjian, H. S., Avas, M.: J. clin. Invest.48, 271 (1969).Google Scholar
  2. 2.
    Gertz, K. H.: Pflügers Arch. ges. Physiol.276, 336 (1963).Google Scholar
  3. 3.
    —, Mangos, J. A., Braun, G., Pagel, H. D.: Pflügers Arch. ges. Physiol.285, 360 (1965).Google Scholar
  4. 4.
    Györy, A. Z.: Pflügers Arch.319, R 77 (1970).Google Scholar
  5. 5.
    Hayslett, J. P., Kashgarian, M., Epstein, F. H.: J. clin. Invest.47, 774 (1968).Google Scholar
  6. 6.
    Morel, F., Murayama, Y.: Pflügers Arch.320, 1 (1970).Google Scholar
  7. 7.
    Morgan, T., Berliner, R. W.: Amer. J. Physiol.217, 992 (1969).Google Scholar
  8. 8.
    Nakajima, K., Clapp, J. R., Robinson, R. R.: Amer. J. Physiol.219, 345 (1970).Google Scholar
  9. 9.
    Radtke, H. W., Rumrich, G., Klöss, S., Ullrich, K. J.: Pflügers Arch.319, R 78 (1970).Google Scholar
  10. 10.
    Schnermann, J., Levine, D. Z., Horster, M.: Pflügers Arch.308, 149 (1969).Google Scholar
  11. 11.
    Snedecor, G. W., Cochran, W. G.: Statistical methods. 6th Edition. Iowa: Iowa State University Press 1967.Google Scholar
  12. 12.
    Steinhausen, M.: Pflügers Arch. ges. Physiol.298, 105 (1967).Google Scholar
  13. 13.
    —, Iravani, F., Schubert, G. E., Taugner, R.: Virchows Arch. path. Anat.336, 503 (1963).Google Scholar
  14. 14.
    Ullrich, K. J.: Permeability characteristics of the mammalian nephron. In: Handbook of physiology. Executive Editor: Stephan R. Geiger. Baltimore: The Williams and Wilkins Company (in press).Google Scholar
  15. 15.
    —, Frömter, E., Baumann, K.: Micropuncture and microanalysis in kidney physiology. In: Laboratory techniques in membrane biophysics. Edited by H. Passow and R. Stämpfli. Berlin-Heidelberg-New York: Springer 1969.Google Scholar
  16. 16.
    Wiederholt, M., Langer, K. H., Thoenes, W., Hierholzer, K.: Pflügers Arch.302, 166 (1968).Google Scholar
  17. 17.
    Wright, F. S., Howards, S. S., Knox, F. G., Berliner, R. W.: Amer. J. Physiol.217, 199 (1969).Google Scholar

Copyright information

© Springer-Verlag 1971

Authors and Affiliations

  • A. Z. Györy
    • 1
  1. 1.Max-Planck-Institut für BiophysikFrankfurt/M. 70Germany

Personalised recommendations