Summary
A sucrose-gap chamber is described which is particularly suitable for measuring potential changes in large smooth muscle preparations, of length about 20 mm and cross-sectional area up to 1 mm2. The sucrose section of the measuring canal is separated from the Krebs and KCl solutions by rubber membranes with a central hole whose diameter can be regulated by concentric pressure on the rubber membrane. Liquid junction potentials between the different solutions can thereby be eliminated. Tests with taenia coli and stomach muscle from the guinea-pig have shown that the values obtained for membrane potential and slow potential fluctuations approximate closely to those obtained by intracellular measurements. In addition, good stability was found in long-term recordings.
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Supported by the Deutsche Forschungsgemeinschaft (Go 130/17).
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Boev, K., Golenhofen, K. Sucrose-gap technique with pressed-rubber membranes. Pflugers Arch. 349, 277–283 (1974). https://doi.org/10.1007/BF00592455
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DOI: https://doi.org/10.1007/BF00592455