Summary
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1.
Lactate turnover was measured in 24 anesthetized rats using14C-U-(l) Lactate with the priming dose-infusion technique.
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2.
14C Lactate in blood was determined with a specific radio-enzymatic micromethod.
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3.
Lactate turnover increases with blood lactate concentration in the range 0.5 to 3 mM. In a steady state system this relationship means that a new equilibrium may be achieved at a higher pool level, between lactate production and utilization.
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4.
Comparing available data in four mammalian species lactate turnover at normal lactate levels was found to be approximately constant when computed on a body surface unit basis.
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Adolph, E. F.: Quantitative relation in the physiological constitution of mammals. Science109, 579–585 (1949).
Altman, P. L., Dittmer, D. S.: Metabolism. Biological Handbook. Federation of American societies of experimental Biology. Bethesda, Maryland 1968.
Annison, E. F., Lindsay, D. B., White, R. R.: Metabolic interrelation of glucose and lactate in sheep. Biochem. J.88, 243–248 (1963).
Baumann, R., Bauer, Ch., Bartels, H.: Influence of chronic and acute hypoxia on oxygen affinity and red cells 2–3 diphosphoglycerate of rats and guinea pigs. Resp. Physiol.11, 135–144 (1971).
Bergmeyer: Methods of enzymatic analysis. Ed. Bergmeyer. Academic press 1965.
Cain, S. M.: Effect of PCO2 on the relation of lactate and excess lactate to O2 deficit. Amer. J. Physiol.214, 1322–1327 (1968).
Depocas, F., Minaire, Y., Chatonnet, J.: Rates of formation and oxidation of lactic acid in dogs at rest and during moderate exercise. Canad. J. Physiol.47, 603–610 (1963).
Doar, J. N. H., Gramp, D. G.: The effects of obesity and maturity-onset diabetes mellitus onl(−) Lactic acid metabolism. Clin. Sci.39, 271–279 (1970).
Drury, D. R., Wick, A. N.: Metabolism of lactic acid in the intact rabbit. Amer. J. Physiol.184, 304–308 (1956).
——: Chemistry and metabolism ofl(+) andd(−) lactic acids. Ann. N. Y. Acad. Sci.119, 1061–1069 (1965).
Forbath, N., Kenshole, A. B., Hetenyi, G., Jr.: Turnover of lactic acid in normal and diabetic dogs calculated by two tracer methods. Amer. J. Physiol.213, 1179–1184 (1967).
Freminet, A., Bursaux, E., Poyart, C.: An improved micromethod for the determination of L. Lactate14C in blood. Biochem. Med.6, 72–76 (1972).
Garcia, A. C., Lai, Y. L., Attebery, B. A., Brown, E. B., Jr.: Lactate and pyruvate accumulation during hypocapnia. Resp. Physiol.12, 371–380 (1971).
Hill, A. V., Long, C. N. H., Lupton, H.: Muscular exercise, lactic acid and the supply and utilization of oxygen. Proc. roy. Soc. B96 438 (1924).
Huckabee, N. E.: Relationship of pyruvate and lactate during anaerobic metabolism. I. Effect of infusions of pyruvate or glucose and of hyperventilation. J. clin. Invest.37, 244–254 (1958).
Kazemi, H., Valencia, L. M., Shannon, D. C.: Brain and cerebrospinal fluid lactate concentration in respiratory acidosis and alkalosis. Resp. Physiol.6, 178–186 (1969).
Kleiber, M.: Body size and metabolic rate. Physiol. Rev.27, 511–541 (1947).
Kreisberg, R. A., Pennington, L. F., Bashell, C. R.: Lactate turnover and glucogenesis in normal and obese humans. Effect of starvation. Diabetes19, 53–63 (1970).
Lie, S. E., Loken, A. C., Stromme, J. H.: Fatal congenital lactic acidosis in two siblings. Acta ped. scand.60, 129–137, 138–145 (1971).
Meeh, K.: Oberflächenmessungen des menschlichen Körpers. Z. Biol. 425–458 (1879).
Scholander, P. F.: Analysis for accurate estimation of respiratory gases in one half cubic centimeter samples. J. biol. Chem.167, 235–250 (1947).
Schwartz, D.: Methodes statistiques à l'usage des médecins et des biologistes. Méd. Flammarion 1963.
Steele, R.: Reflections on pools. Fed. Proc.23, 671–679 (1964).
Steele, R.: Tracer probes in steady state systems, vol. 1. Springfield, Ill.: Ch. C. Thomas.
—, Wall, S. S., de Bodo, R. C., Altszuler, N.: Measurement of size and turnover rate of body glucose pool by the isotope dilution method. Amer. J. Physiol.187, 15–24 (1956).
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Freminet, A., Bursaux, E. & Poyart, C.F. Mesure de la vitesse de renouvellement du lactate chez le rat par perfusion de 14-C-U (l) lactate. Pflugers Arch. 334, 293–302 (1972). https://doi.org/10.1007/BF00592164
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DOI: https://doi.org/10.1007/BF00592164