Roux's archives of developmental biology

, Volume 201, Issue 5, pp 275–283 | Cite as

A study on cell lineage, especially the germ cell line, in embryos of the teleost fish,Barbus conchonius

  • Petra Gevers
  • John Dulos
  • Jos G. M. van den Boogaart
  • Lucy P. M. Timmermans
Original Articles


Lucifer Yellow-Dextran labelling of lower layer cells (LLC), sometimes together with upper layer cells (ULC), of the 64-cellBarbus conchonius embryo resulted in labelled primordial germ cells (PGCs) at 12 h after fertilization (a.f.) in about 25% of cases. The presence of labelled PGCs was independent of the location of the injected blastomere with respect to the later orientation of the embryonic axis. After injection of an ULC alone, however, labelled PGCs were never found. Also, the distribution of labelled somatic cells differed between the ULC- and LLC-injected embryos. When we found fluorescent PGCs, only a few of them were labelled, suggesting that either a single predecessor exists earlier than the 64-cell stage or that the formation of germ cells is a polyclonal process. Tracing the fluorescent cells at successive stages of development shows an extensive mixing with unlabelled cells during the epiboly stage, which might well be the cause of partly unpredictable cell lineages. The chance of being committed to a specific fate is different for the ULC and LLC descendants. This might be due to relatively limited cell mixing between these two cell populations.

Key words

Cell lineage Primordial germ cell Fish development 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Ballard WW (1973) Morphogenetic movements inSalmo gairdneri. J Exp Zool 184:27–48Google Scholar
  2. Ballard WW (1982) Morphogenetic movements and fate map of the cyprinoform teleost,Catostomus commersoni (Lacepede). J Exp Zool 219:301–321Google Scholar
  3. Copp AJ, Roberts HM, Polani PE (1986) Chimaerism of primordial germ cells in the early postimplantation mouse embryo following microsurgical grafting of posterior primitive streak cellsin vitro. J Embryol Exp Morphol 95:95–115Google Scholar
  4. Dixon KE (1981) The origin of the primordial germ cells in the amphibia. Neth J Zool 31:5–37Google Scholar
  5. Eddy EM (1984) Origin of the germ cell line. In: Blerkom J van, Motta PM (eds) Ultrastructure of reproduction. Martinus Nijhof Publishers, Den Haag, pp 1–12Google Scholar
  6. Eyal-Giladi H, Ginsburg M, Farberov A (1981) Avian primordial germ cells are of epiblastic origin. J Embryol Exp Morphol 65:139–147Google Scholar
  7. Gevers P, Timmermans LPM (1991) Dye-coupling and the formation and fate of the hypoblast in the teleost fish embryo,Barbus conchonius. Development 112:431–438Google Scholar
  8. Kageyama T (1977) Motility and locomotion of embryonic cells of the medaka,Oryzias latipes, during early development. Dev Growth Differ 19:103–110Google Scholar
  9. Kimmel CB, Law RD (1985a) Cell lineage of zebrafish blastomeres: I. Cleavage pattern and cytoplasmic bridges between cells. Dev Biol 108:78–85Google Scholar
  10. Kimmel CB, Law RD (1985b) Cell lineage of zebrafish blastomeres: II. Formation of the yolk syncytial layer. Dev Biol 108:86–93Google Scholar
  11. Kimmel CB, Law RD (1985c) Cell lineage of zebrafish blastomeres: III. Clonal analysis of the blastula and gastrula stages. Dev Biol 108:94–101Google Scholar
  12. Kimmel CB, Warga RM (1986) Tissue specific cell lineage originate in the gastrula of the zebrafish. Science 231:365–368Google Scholar
  13. Kimmel CB, Warga RM (1987) Indeterminate cell lineage of the zebrafish embryo. Dev Biol 124:269–280Google Scholar
  14. Kimmel CB, Warga RM (1988) Cell lineage and developmental potential of cells in the zebrafish embryo. Trends Genet 3:68–73Google Scholar
  15. Kimmel CB, Warga RM, Schilling TF (1990) Origin and organization of the zebrafish fate map. Development 108:581–594Google Scholar
  16. Lesseps R, Hall M, Murnand MB (1979) Contact inhibition of cell movement in living embryos of an annual fish,Notobranchus korthausae. J Exp Zool 207:459–470Google Scholar
  17. McLaren A (1983) Primordial germ cells in mice. Biblthca Anat 24:59–66Google Scholar
  18. Moody SA (1987) Fates of the blastomeres of the 16 cell stageXenopus embryo. Dev Biol 119:560–578Google Scholar
  19. Nieuwkoop PD, Sutasurya LA (1976) Embryological evidence for a possible polyphyletic origin of the recent amphibians. J Embryol Exp Morphol 35:159–167Google Scholar
  20. Nieuwkoop PD, Sutasurya LA (1979) Primordial germ cells in the chordates; Embryogenesis and phylogenesis. Cambridge University Press, Cambridge London New York Melbourne, pp 1–187Google Scholar
  21. Timmermans LPM, Taverne N (1989) Segregation of primordial germ cells, their numbers and their fates during early development ofBarbus conchonius L. (Cyprinidae, Teleostei). A study with3H-thymidine incorporation. J Morphol 202:225–237Google Scholar
  22. Trinkaus JP, Lentz TL (1967) Surface specialization ofFundulus cells and their relation to cell movement during gastrulation. J Cell Biol 32:139–153Google Scholar
  23. Wal UP van der, Dohmen MR (1978) A method for the orientation of small and delicate objects in embedding media for light and electron microscopy. Stain Techn 53:56–58Google Scholar
  24. Walker C, Streisinger G (1983) Induction of mutations by X-rays in pregonial germ cells of zebrafish embryos. Genetica 103:125–136Google Scholar
  25. Warga RM, Kimmel CB (1990) Cell movements during epiboly and gastrulation in zebrafish. Development 108:569–580Google Scholar
  26. Wood A, Timmermans LPM (1988) Teleost epiboly: a reassessment of deep cell movement in the germ ring. Development 102:575–585Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Petra Gevers
    • 1
  • John Dulos
    • 1
  • Jos G. M. van den Boogaart
    • 1
  • Lucy P. M. Timmermans
    • 1
  1. 1.Department of Animal Morphology and Cell BiologyAgricultural UniversityWageningenThe Netherlands

Personalised recommendations