Roux's archives of developmental biology

, Volume 201, Issue 5, pp 275–283 | Cite as

A study on cell lineage, especially the germ cell line, in embryos of the teleost fish,Barbus conchonius

  • Petra Gevers
  • John Dulos
  • Jos G. M. van den Boogaart
  • Lucy P. M. Timmermans
Original Articles

Summary

Lucifer Yellow-Dextran labelling of lower layer cells (LLC), sometimes together with upper layer cells (ULC), of the 64-cellBarbus conchonius embryo resulted in labelled primordial germ cells (PGCs) at 12 h after fertilization (a.f.) in about 25% of cases. The presence of labelled PGCs was independent of the location of the injected blastomere with respect to the later orientation of the embryonic axis. After injection of an ULC alone, however, labelled PGCs were never found. Also, the distribution of labelled somatic cells differed between the ULC- and LLC-injected embryos. When we found fluorescent PGCs, only a few of them were labelled, suggesting that either a single predecessor exists earlier than the 64-cell stage or that the formation of germ cells is a polyclonal process. Tracing the fluorescent cells at successive stages of development shows an extensive mixing with unlabelled cells during the epiboly stage, which might well be the cause of partly unpredictable cell lineages. The chance of being committed to a specific fate is different for the ULC and LLC descendants. This might be due to relatively limited cell mixing between these two cell populations.

Key words

Cell lineage Primordial germ cell Fish development 

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Petra Gevers
    • 1
  • John Dulos
    • 1
  • Jos G. M. van den Boogaart
    • 1
  • Lucy P. M. Timmermans
    • 1
  1. 1.Department of Animal Morphology and Cell BiologyAgricultural UniversityWageningenThe Netherlands

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