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Regional localization of DNA probes on the short arm of chromosome 11 using aniridia-Wilms' tumor-associated deletions

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Summary

We are interested in the precise localization of various DNA probes on the short arm of chromosome 11 for our research on the aniridia-Wilms' tumor association (AWTA), assigned to region 11p13 (Knudson and Strong 1972; Riccardi et al. 1978). For this purpose we have screened lymphocyte DNA and material derived from somatic cell hybrids from individuals with constitutional 11p deletions with a range of available probes: D11S12; calcitonin/CGRP (CALC1/CALC2); insulin (INS); Harvey ras 1 (HRAS 1); beta-globin gene cluster (HBBC); human insulin-like growth factor 2 (IGF-2); parathyroid hormone (PTH); human pepsinogen A (PGA). Using this material, it has been possible to map all probes used, except insulin, outside the region 11p111-p15.1, resulting in an SRO (same regional overlap) of 11p15.1-p15.5 for most probes. We found an SRO for PGA of 11p111-q12 and an SRO for CALC2 of 11p15.1-p15.5 or 11p111-q12. We have localised the insulin gene to band 11p15.1.

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Authors and Affiliations

  1. Institute of Human Genetics (AMC), University of Amsterdam, Meibergdreef 15, NL-1105 AZ, Amsterdam ZO, The Netherlands

    M. Mannens, R. M. Slater & C. Heyting

  2. Department of Cell Biology and Genetics, Erasmus University, Dijkzigtkamer 816, NL-3000 DR, Rotterdam, The Netherlands

    A. Geurts van Kessel

  3. Abteilung Humangenetik der Universität, Schwanenweg 24, D-2300, Kiel, Federal Republic of Germany

    E. Goedde-Salz

  4. Institute of Human Genetics, Free University of Amsterdam, Postbus 7161, NL-1007 MC, Amsterdam, The Netherlands

    R. R. Frants

  5. Department of Human Genetics, State University of Leiden, Wassenaarseweg 72, NL-2333 AL, Leiden, The Netherlands

    G. J. B. Van Ommen & P. L. Pearson

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  1. M. Mannens
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  2. R. M. Slater
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  3. C. Heyting
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  4. A. Geurts van Kessel
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  5. E. Goedde-Salz
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Mannens, M., Slater, R.M., Heyting, C. et al. Regional localization of DNA probes on the short arm of chromosome 11 using aniridia-Wilms' tumor-associated deletions. Hum Genet 75, 180–187 (1987). https://doi.org/10.1007/BF00591083

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  • DOI: https://doi.org/10.1007/BF00591083

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