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The effect of temperature on Na currents in rat myelinated nerve fibres

  • Jürgen R. Schwarz
Excitable Tissues and Central Nervous Physiology

Abstract

  1. 1)

    Voltage clamp experiments were performed in single myelinated nerve fibres of the rat and the effect of temperature on Na currents was investigated between 0°C and 40°C.

     
  2. 2)

    The amplitude of the peak Na current changed with aQ 10=1.1 between 40° and 20°C and with aQ 10=1.3 between 20° and 10°C. Below 10°C the peak Na current changed with aQ 10=1.9.

     
  3. 3)

    The temperature coefficient for time-to-peak (tp), the measure for Na activation, and τh1 and τh2, the time constants for Na inactivation changed throughout the temperature range.Q 10 for all of these kinetic parameters increased from 1.8–2.1 between 40° and 20°C to 2.6–2.7 between 20° and 10°C. Below 10°CQ 10 increased to 3.7 for τh1 and tp, and to 2.9 for τh2. When the series resistance artifacts were minimized by addition of 6 nM TTX, theQ 10's atT<10°C were 2.9–3.0.

     
  4. 4)

    When the temperature was decreased from 20° to 0°C, both the curve relating Na permeability to potential,P Na(V), and the steady state Na inactivation curve,h∞(V), were reversibly shifted towards more negative potentials by 6 mV and 11 mV, respectively. When the temperature was increased from 20° to 37°C no shifts occurred.

     
  5. 5)

    The Hodgkin-Huxley rate constants αh(V) and βh(V) were calculated fromh (V) and τh (or τh1) at 20° and 4°C. The shift inh∞(V) and the change inh (V), which occurred within this temperature range, could be described assuming a selective shift in τh(V) along the potential axis.

     

Key words

Node of Ranvier Rat nerve Na current Temperature dependence Voltage clamp 

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References

  1. Aldrich RW, Corey DP, Stevens CF (1983) A reinterpretation of mammalian sodium channel gating based on single channel recording. Nature (Lond) 306:436–441CrossRefGoogle Scholar
  2. Benoit E, Corbier A, Dubois J-M (1985) Evidence for two transient sodium currents in the frog node of Ranvier. J Physiol (Lond) 361:339–360Google Scholar
  3. Brismar T (1980) Potential clamp analysis of membrane currents in rat myelinated nerve fibres. J Physiol (Lond) 298:171–184Google Scholar
  4. Brismar T, Schwarz JR (1985) Potassium permeability in rat myelinated nerve fibres. Acta Physiol (Scand) 124:141–148CrossRefGoogle Scholar
  5. Chapman D (1975) Phase transitions and fluidity characteristics of lipids and cell membranes. Quart Rev Biophys 8:185–235CrossRefGoogle Scholar
  6. Chiu SY (1976) Inactivation of sodium channels: second order kinetics in myelinated nerve. J Physiol (Lond) 273:573–596Google Scholar
  7. Chiu SY, Mrose HE, Ritchie JM (1979) Anomalous temperature dependence of sodium conductance in rabbit nerve compared with frog nerve. Nature (Lond) 279:327–328CrossRefGoogle Scholar
  8. Chiu SY, Ritchie JM, Rogart RB, Stagg D (1979) A quantitative description of membrane currents in rabbit myelinated nerve fibres. J Physiol (Lond) 292:149–166Google Scholar
  9. Cole KS (1968) Membranes, ion and impulses, University of California Press, Berkeley Los AngelesGoogle Scholar
  10. Courtney KR (1979) Extracellular pH selectively modulates recovery from sodium inactivation in frog myelinated nerve. Biophys J 28:363–368PubMedCrossRefGoogle Scholar
  11. Drouin H, Neumcke B (1974) Specific and unspecific charges at the sodium channels of the nerve membrane. Pflügers Arch 351:207–229PubMedCrossRefGoogle Scholar
  12. Dudel J, Rüdel R (1970) Voltage and time dependence of excitatory sodium current in cooled sheep Purkinje fibres. Pflügers Arch 313:136–158CrossRefGoogle Scholar
  13. Fischbach GD, Lass Y (1978) A transition temperature for acetylcholine channel conductance in chick myoballs. J Physiol (Lond) 280:527–536Google Scholar
  14. Frankenhaeuser B (1960) Sodium permeability in toad nerve and in squid nerve. J Physiol (Lond) 152:159–166Google Scholar
  15. Frankenhaeuser B, Moore LE (1963) The effect of temperature on the sodium and potassium permeability changes in myelinated nerve fibres ofXenopus laevis. J Physiol (Lond) 169:431–437Google Scholar
  16. Goldman L, Schauf CL (1972) Inactivation of the sodium current in Myxicola giant axons. J Gen Physiol 59:659–675PubMedCrossRefGoogle Scholar
  17. Harper AA, MacDonald AG, Wann KT (1983) The effect of temperature on the nerve-blocking action of benzyl alcohol on the squid giant axon. J Physiol (Lond) 338:51–60Google Scholar
  18. Haydon DA, Kimura JE (1981) Some effects of n-pentane on the sodium andpotassium currents of the squid giant axon. J Physiol (Lond) 338:57–70Google Scholar
  19. Haydon DA, Urban BW (1983) The action of hydrocarbons and carbon tetrachloride on the sodium current of the squid. J Physiol (Lond) 338:435–450Google Scholar
  20. Haydon DA, Requena J, Urban BW (1980) Some effects of aliphatic hydrocarbons on the electrical capacity and ionic currents of the squid giant axon membrane. J Physiol (Lond) 309:229–245Google Scholar
  21. Hodgkin AL, Huxley AF (1952) A quantitative description of membrane current and its application to conduction and excitation in nerve. J Physiol (Lond) 117:500–544Google Scholar
  22. Horáckova M, Nonner W, Stämpfli R (1968) Action potentials and voltage clamp currents of single rat Ranvier nodes. Pro Int Union Physiol Sci 7:198Google Scholar
  23. Kimura JE, Meves H (1979) The effect of temperature on the asymmetrical charge movement in squid giant axons. J Physiol (Lond) 289:479–500Google Scholar
  24. Kniffki K-D, Siemen D, Vogel W (1981) Development of sodium permeability inactivation in nodal membranes. J Physiol (Lond) 313:37–48Google Scholar
  25. Luzzati V (1968) X-ray diffraction studies of lipid-water systems. In: Chapman D (ed) Biological membranes. Academic Press, Orlando, pp 118–119Google Scholar
  26. Moore LE (1971) The effect of temperature and calcium ions on the rate constants of myelinated nerve. Am J Physiol 221:131–137PubMedGoogle Scholar
  27. Mrose HE, Chiu SY (1979) Is the steady-state inactivation curve for the sodium channel temperature dependent? Biophys J 25:193aGoogle Scholar
  28. Netter H (1959) Theoretische Biochemie. Springer, Berlin Göttingen Heidelberg, p 557CrossRefGoogle Scholar
  29. Neumcke B, Stämpfli R (1982) Sodium currents and sodium-dependent fluctuations in rat myelinated nerve fibres. J Physiol (Lond) 329:163–184Google Scholar
  30. Neumcke B, Stämpfli R (1983) Alteration of the conductance of Na channels in the nodal membrane of frog nerve by holding potential and tetrodotoxin. Biochim Biophys Acta 727:177–184PubMedCrossRefGoogle Scholar
  31. Nonner W (1969) A new voltage clamp method for Ranvier nodes. Pflügers Arch 309:176–192PubMedCrossRefGoogle Scholar
  32. Ochs G, Bromm B, Schwarz JR (1981) A three-state model for inactivation of sodium permeability. Biochim Biophys Acta 645:243–252PubMedCrossRefGoogle Scholar
  33. Paintal AS (1965) Block of conduction in mammalian myelinated nerve fibres by low temperatures. J Physiol (Lond) 180:1–19Google Scholar
  34. Schauf CL (1973) Temperature dependence of ionic currents kinetics of Myxicola giant axons. J Physiol (Lond) 235:197–205Google Scholar
  35. Schwarz JR (1984a) Rat myelinated nerve fibres exhibit a transition temperature for Na permeability properties. Pflügers Arch 400:R37Google Scholar
  36. Schwarz JR (1984b) Different temperature coefficients of the rate constants αh and βh in rat nerve fibres. Pflügers Arch 402:R32Google Scholar
  37. Schwarz JR, Bromm B, Ochs G (1980) Phenobarbital induces slow recovery from sodium inactivation of the nodal membrane. Biochim Biophys Acta 597:384–390PubMedCrossRefGoogle Scholar
  38. Schwarz JR, Neumcke B, Stämpfli R (1985) There is no difference in Na inactivation between rat and frog myelinated nerve. Pflügers Arch 405:R 51Google Scholar
  39. Schwarz W (1979) Temperature experiments on nerve and muscle membranes of frogs. Indications for a phase transition. Pflügers Arch 382:27–34PubMedCrossRefGoogle Scholar
  40. Sigworth FJ (1980) The variance of sodium current fluctuations at the node of Ranvier. J Physiol (Lond) 307:97–129Google Scholar
  41. Singer SJ, Nicholson GL (1972) The fluid mosaic model of the structure of all membranes. Science 175:720–731PubMedCrossRefGoogle Scholar
  42. Stämpfli R, Hille B (1976) Electrophysiology of the peripheral nerve. In: Llinas R, Precht W (eds) Handbook of frog neurobiology. Springer, Berlin Heidelberg New York, pp 3–32CrossRefGoogle Scholar
  43. Wang CM, Narahashi T, Scuka M (1972) Mechanism of the negative temperature coefficient of nerve blocking action of allethrin. J Pharmacol Exp Ther 182:442–453PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Jürgen R. Schwarz
    • 1
  1. 1.Physiologisches Institut der Universität HamburgUKEHamburg 20Federal Republic of Germany

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