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Electrical properties of renal collecting duct principal cell epithelium in tissue culture

  • Peter Gross
  • Will W. Minuth
  • Wilhelm Kriz
  • Eberhard Frömter
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands

Summary

Whereas collecting duct epithelium in vivo is composed of principal and intercalated cells, we grew a pure principal cell epithelium using a new technique involving tissue culture. These principal cells were derived from collecting duct anlagen of newborn rabbits. We investigated the electrical properties of such epithelia in a newly designed lucite double-chamber with an inner opening of 0.08 cm2. Our observations were: 1) mean transepithelial resistanceR te was 0.83±0.2 kΩcm2 at 37° C and after preincubation in aldosterone; 2) mean transepithelial potential differenceV te was low and variable under standard conditions and at room temperature but increased to −59.5±4.4 mV (sign referring to polarity of apical surface) after preincubation in 10−6 mol/l aldosterone and at 37° C; 3) 10−6 mol/l amiloride added to the apical perfusion fluid largely abolished thisV te while increasingR te by 120%; 4) experiments with 5×10−3 mol/l BaCl2 in the apical perfusion fluid failed to changeR te andV te significantly. This principal cell epithelium therefore has characteristics of a “tight” epithelium with active sodium transport; however, its electrical properties differ from those of the isolated perfused collecting duct segment.

Key words

Collecting duct Principal cell Tissue culture Aldosterone Amiloride Sodium transport 

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Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • Peter Gross
    • 1
  • Will W. Minuth
    • 2
  • Wilhelm Kriz
    • 2
  • Eberhard Frömter
    • 3
  1. 1.Medizinische UniversitätsklinikUniversität HeidelbergHeidelbergFederal Republic of Germany
  2. 2.Anatomie IUniversität HeidelbergHeidelbergFederal Republic of Germany
  3. 3.Zentrum der PhysiologieFrankfurt/M. 70Federal Republic of Germany

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