Whereas collecting duct epithelium in vivo is composed of principal and intercalated cells, we grew a pure principal cell epithelium using a new technique involving tissue culture. These principal cells were derived from collecting duct anlagen of newborn rabbits. We investigated the electrical properties of such epithelia in a newly designed lucite double-chamber with an inner opening of 0.08 cm2. Our observations were: 1) mean transepithelial resistanceRte was 0.83±0.2 kΩcm2 at 37° C and after preincubation in aldosterone; 2) mean transepithelial potential differenceVte was low and variable under standard conditions and at room temperature but increased to −59.5±4.4 mV (sign referring to polarity of apical surface) after preincubation in 10−6 mol/l aldosterone and at 37° C; 3) 10−6 mol/l amiloride added to the apical perfusion fluid largely abolished thisVte while increasingRte by 120%; 4) experiments with 5×10−3 mol/l BaCl2 in the apical perfusion fluid failed to changeRte andVte significantly. This principal cell epithelium therefore has characteristics of a “tight” epithelium with active sodium transport; however, its electrical properties differ from those of the isolated perfused collecting duct segment.
Collecting duct Principal cell Tissue culture Aldosterone Amiloride Sodium transport
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