Pflügers Archiv

, Volume 390, Issue 2, pp 207–210 | Cite as

Voltage clamp of single freshly dissociated smooth muscle cells: Current-voltage relationship for three currents

  • John V. WalshJr.
  • Joshua J. Singer
Excitable Tissues and Central Nervous Physiology Letters and Notes

Abstract

Voltage-clamp experiments on single freshly dissociated (i.e. uncultured) vertebrate smooth muscle cells were carried out under conditions where the inital inward current, as well as various phases of outward current, could be studied. Current-voltage relationships were obtained for the initial current, the peak outward current, and a later, steady-state outward current, over a potential range of approximately −130 mV to +50 mV. Evidence is presented that the initial current is carried by Ca++ ions and is responsible for the rising phase of the action potential and that the peak in the outward current is due to Ca++ activation of K+ conductance.

Key words

Voltage clamp Calcium current Dissociated smooth muscle cell 

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References

  1. 1.
    Aldrich, R.W. Jr., Getting, P.A., Thompson, S.H.: Inactivation of delayed outward current in molluscan neurone somata. J. Physiol. 291:507–530 (1979).Google Scholar
  2. 2.
    Anderson, N.C., Jr.: Limitations and possibilities in smooth muscle voltage-clamp. In: Casteels, R., Godfraind, T., Ruegg, J.C. (eds.)Excitation-Contraction Coupling in Smooth Muscle. Elsevier, New York, pp. 81–89 (1977).Google Scholar
  3. 3.
    Bagby, R.M., Young, A.M., Dotson, R.S., Fisher, B.A., and McKinnon, K.: Contraction of single smooth muscle cells fromBufo marinus stomach. Nature London 234:351–352 (1971).Google Scholar
  4. 4.
    Bennett, M.R.:Autonomic Neuromuscular Transmission. London: Cambridge (1972).Google Scholar
  5. 5.
    Caffrey, J.M., Anderson, N.C., Jr., and Moore, J.W.: Voltage clamp of single isolated smooth muscle cells. Federation Proc. 39:2077 (1980).Google Scholar
  6. 6.
    Coburn, R.F., Ohba, M., Tomita, T.: Recording of intracellular electrical activity with the voltage-clamp technique with double sucrose gap. In: Daniel, E.E., Paton, D.M., (eds.)Methods In Pharmacology. Vol. 3 Smooth Muscle, Plenum, New York p. 247–260 (1975).Google Scholar
  7. 7.
    Colatsky, T.J., Tsien, R.W.: Sodium channels in rabbit cardiac Purkinje fibres. Nature 278:265–268 (1979).Google Scholar
  8. 8.
    Eckert, R., Tillotson, D.: Potassium activation associated with intraneuronal free calcium. Science 200:437–439 (1978).Google Scholar
  9. 9.
    Inomata, H., Kao, C.Y.: Ionic mechanisms of repolarization in the guinea-pig taenia coli as revealed by the actions of strontium. J. Physiol. 297:443–462 (1979).Google Scholar
  10. 10.
    Johnson, E.A., Lieberman, M.: Heart: excitation and contraction. Ann. Rev. Physiol. 33:479–532 (1971).Google Scholar
  11. 11.
    Meech, R.W., Standen, N.B.: Potassium activation inHelix aspersa neurones under voltage clamp: a component mediated by calcium influx. J. Physiol. 249:211–239 (1975).Google Scholar
  12. 12.
    Mironneau, J., Savineau, J.P., Rahety, A.: Evidence for a component of the outward current mediated by calcium influx in uterine smooth muscle. In: Casteels, R., Godfraind, T., Ruegg, J.C. (eds.)Excitation-Contraction Coupling in Smooth Muscle, Elsevier, New York, pp. 117–122 (1977).Google Scholar
  13. 13.
    Singer, J.J., Fay, F.S.: Detection of contraction of isolated smooth muscle cells in suspension. Am. J. Physiol. 232 (Cell Physiol. 1): C138-C143 (1977).Google Scholar
  14. 14.
    Singer, J.J., Walsh, J.V., Jr.: Passive properties of the membrane of single freshly isolated smooth muscle cells. Am. J. Physiol. 239 (Cell Physiol. 8): C153-C161 (1980).Google Scholar
  15. 15.
    Singer, J.J., Walsh, J.V., Jr.: Rectifying properties of the membrane of single freshly isolated smooth muscle cells. Am. J. Physiol. 239 (Cell Physiol. 8): C175-C181 (1980).Google Scholar
  16. 16.
    Singer, J.J., Walsh, J.V., Jr.: Membrane currents of single, freshly isolated smooth muscle cells studied with voltage clamp. Soc. Neurosci. Abstr. 6:837 (1980).Google Scholar
  17. 17.
    Singer, J.J., Walsh, J.V., Jr.: Four ionic currents in single freshly isolated smooth muscle cells studied with voltage clamp and microperfusion. Biophys. J. 33:37a (1981).Google Scholar
  18. 18.
    Tomita, T.: Electrical properties of mammalian smooth muscle. In: Bulbring, E., Brading, A.F., Jones, A.W., Tomita, T. (eds)Smooth Muscle. Williams and Wilkins, Baltimore pp. 197–243. (1970).Google Scholar
  19. 19.
    Tomita, T.: Electrophysiology of mammalian smooth muscle. Prog. Biophys. Mol. Biol. 30:185–203 (1975).Google Scholar
  20. 20.
    Walsh, J.V., Jr., Singer, J.J.: Calcium action potentials in single freshly isolated smooth muscle cells. Am. J. Physiol. 239 (Cell Physiol. 8): C162-C174 (1980).Google Scholar
  21. 21.
    Walsh, J.V., Jr., Singer, J.J.: Penetration-induced hyperpolarization as evidence for Ca2+ activation of K+ conductance in isolated smooth muscle cells. Am. J. Physiol. 239 (Cell Physiol. 8):C182-C189 (1980).Google Scholar
  22. 22.
    Walsh, J.V. Jr., Singer, J.J.: Voltage clamp of single freshly-isolated smooth muscle cells. Federation Proc. 39:2078 (1980).Google Scholar
  23. 23.
    Weigel, R.J., Connor, J.A., Prosser, C.L.: Two roles of calcium during the spike in circular muscle of small intestine in cat. Am. J. Physiol. 237 (Cell Physiol. 6): C247-C256 (1979).Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • John V. WalshJr.
    • 1
  • Joshua J. Singer
    • 1
  1. 1.Department of PhysiologyUniversity of Massachusetts Medical SchoolWorcesterUSA

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