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Inactivation of allantoinase ofPseudomonas aeruginosa in vivo

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Abstract

The total and specific activity of allantoinase decreased in the stationary growth phase whenPseudomonas aeruginosa was cultured in a medium containing allantoin or allantoate as the sole source of carbon, nitrogen and energy. The enzyme was not instableper se. Inactivation was proven to be due to the synthesis of a protein, which exhibited no proteolytic activity toward allantoinase. The synthesis of a specific binding protein is postulated.

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Abbreviations

A600 :

optical density measured at 600 nm

CAP:

chloramphenicol

MB:

the inorganic portion of medium A

TEA:

triethanolamine

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Authors and Affiliations

  1. Laboratory of Microbiology, Faculty of Science, University of Nijmegen, Nijmegen

    V. F. M. Rijnierse & C. van der Drift

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  1. V. F. M. Rijnierse
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  2. C. van der Drift
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Rijnierse, V.F.M., van der Drift, C. Inactivation of allantoinase ofPseudomonas aeruginosa in vivo . Arch. Microbiol. 96, 319–328 (1974). https://doi.org/10.1007/BF00590187

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  • DOI: https://doi.org/10.1007/BF00590187

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