Abstract
Intracytoplasmic membranes of wild type strain 37 b 4 and mutant strains A1a car-bchl-, A1a car-bchl+ ofRhodopseudomonas capsulata were isolated. The membrane proteins were solubilized and separated by polyacrylamide gel electrophoresis (methods of Takayamaet al., 1964; Weber and Osborn, 1969). The band patterns were compared with each other.
From the strain A1a car-bchl+ reaction center particles were isolated by treatment of membrane with Triton X-100 followed by sucrose density gradient centrifugation. The reaction center particles were found to be enriched in reaction center bacteriochlorophyll. This pigment shows a reversible bleaching at 855 nm and a blue shift at 798 nm. The light harvesting bacteriochlorophyll portion of this fraction was 14–22% of the total bacteriochlorophyll content. The three main proteins of the reaction center particles amount to about 80% of the total protein of the particles. The molecular weights of the main proteins were estimated to be 32000, 27500 and 22500 daltons.
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Abbreviations
- bchl:
-
bacteriochlorophyll
- OD:
-
optical density
- RC:
-
reaction center
- SDS:
-
Sodium dodecyl sulfate
References
Beugeling, T., Slooten, L., Barelds-van de Beek P. G. M. M.: Thin layer chromatography of pigments from reaction center particles ofRps. spheroides. Biochim. biophys. Acta (Amst.)283, 328–333 (1972)
Clayton, R. K.: Spectroscopic analysis of bacteriochlorophyllsin vitro andin vivo. Photochem. Photobiol.5, 669–677 (1966)
Clayton, R. K., Clayton, B. B.: Relations between pigments and proteins in the photosynthetic membranes ofRhodopseudomonas spheroides. Biochim. biophys. Acta (Amst.)283, 492–504 (1972)
Clayton, R. K., Haselkorn, R.: Protein components of bacterial photosynthetic membranes. J. molec. Biol.68, 97–105 (1972)
Clayton, R. K., Wang, R. T.: Photochemical reaction center fromRhodopseudomonas spheroides. In: Methods in enzymology, vol. XXIII, pp. 696–704. New York: Academic Press 1971
Drews, G.: Die Isolierung schwefelfreier Purpurbakterien. Zbl. Bakt., I. Abt. Orig., Suppl.1, 170–178 (1965)
Drews, G., Lampe, H. H., Ladwig, R.: Die Entwicklung des Photosyntheseapparates in Dunkelkulturen vonRhodopseudomonas capsulata. Arch. Mikrobiol.65, 12–28 (1969)
Drews, G., Leutiger, I., Ladwig, R.: Production of protochlorophyll, protopheophytin and bacteriochlorophyll by the mutant A1a ofRhodopseudomonas capsulata. Arch. Mikrobiol.76, 349–363 (1971)
Feher, G.: Some chemical and physical properties of a bacterial reaction center particle and its primary photochemical reactants. Photochem. Photobiol.14, 373–387 (1971)
Fraker, P. J., Kaplan, S.: Isolation and fractionation of the photosynthetic membranous organelles fromRhodopseudomonas spheroides. J. Bact.108, 465–473 (1971)
Fraker, P. J., Kaplan, S.: Isolation and characterization of a bacteriochlorophyll-containing protein fromRhodopseudomonas spheroides. J. biol. Chem.247, 2732–2737 (1972)
Jolchine, G., Reiss-Husson, F., Kamen, M. D.: Active center fractions fromRhodopseudomonas spheroides strain Y. Proc. nat. Acad. Sci. (Wash.)64, 650–653 (1969)
Kim, Y. D.: The conformational stability of bacteriochlorophyll-protein complex isolated from a green photosynthetic bacterium. II. The action of detergents on the bacteriochlorophyll-protein complex. Arch. Biochem. Biophys.140, 354–361 (1970)
Lampe, H. H., Drews, G.: Die Differenzierung des Membransystems vonRhodopseudomonas capsulata hinsichtlich seiner photosynthetischen und respiratorischen Funktion. Arch. Mikrobiol.84, 1–19 (1972)
Lampe, H. H., Oelze, J., Drews, G.: Die Fraktionierung des Membransystems vonRhodopseudomonas capsulata und seine Morphogenese. Arch. Mikrobiol.83, 78–94 (1972)
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin Phenol reagent. J. biol. Chem.193, 269–275 (1951)
Noël, H., van Der Rest, M., Gingras, G.: Isolation and partial characterization of a P 870 reaction center complex from wild typeRhodospirillum rubrum. Biochim. biophys. Acta (Amst.)275, 219–230 (1972)
Oelze, J., Biedermann, M., Drews, G.: Die Morphogenese des Photosyntheseapparates vonRhodospirillum rubrum. I. Isolierung und Charakterisierung von zwei Membransystemen. Biochim. biophys. Acta (Amst.)173, 436–447 (1969a)
Oelze, J., Biedermann, M., Freund-Mölbert, E., Drews, G.: Bacteriochlorophyllgehalt und Proteinmuster der Thylakoide vonR. rubrum während der Morphogenese des Photosyntheseapparates. Arch. Mikrobiol.66, 154–165 (1969b)
Oelze, J., Drews, G.: Untersuchungen über die Membranproteine beiRhodospirillum rubrum. Arch. Mikrobiol.69, 12–19 (1969a)
Oelze, J., Drews, G.: Die Kinetik der Thylakoidsynthese nach Markierung der Membranen mit C2-C14-Azetat. Biochim. biophys. Acta (Amst.)173, 448–455 (1969b)
Oelze, J., Drews, G.: Der Einfluß der Lichtintensität und der Sauerstoffspannung auf die Differenzierung der Membranen vonRhodospirillum rubrum. Biochim. biophys. Acta (Amst.)203, 189–198 (1970a)
Oelze, J., Drews, G.: Variations of NADH oxidase activity and bacteriochlorophyll contents during membrane differentiation inR. rubrum. Biochim. biophys. Acta (Amst.)219, 131–140 (1970b)
Oelze, J., Drews, G.: Membranes of photosynthetic bacteria. Biochim. biophys. Acta (Amst.)265, 209–239 (1972)
Prince, R. C., Crofts, A. R.: Photochemical reaction centers fromRhodopseudomonas capsulata A1a pho+. FEBS Letters35, 213–216 (1973)
Reed, D. W.: Isolation and composition of a photosynthetic reaction center complex fromRps. spheroides. J. biol. Chem.244, 4936–4941 (1969)
Reed, D. W., Peters, G. A.: Characterization of the pigments in reaction center preparations fromRps. spheroides. J. biol. Chem.247, 7148–7152 (1972)
Reed, D. W., Raveed, D., Israel, H. W.: Functional bacteriochlorophyll protein complexes from chromatophores ofRps. spheroides, strain R-20. Biochim. biophys. Acta (Amst.)223, 281–291 (1970)
Reiss-Husson, F., Jolchine, G.: Purification and properties of a photosynthetic reaction center isolated from various chromatophore fractions ofRhodopseudomonas spheroides. Biochim. biophys. Acta (Amst.)256, 440–451 (1972)
Segen, J. B., Gibson, K. D.: Deficiencies of chromatophore proteins in some mutants ofRhodopseudomonas spheroides with altered carotenoids. J. Bact.105, 701–709 (1971)
Slooten, L.: Reaction center preparations ofRhodopseudomonas spheroides. Energy transfer and structure. Biochim. biophys. Acta (Amst.)256, 452–466 (1972)
Smith, J. H. C., Benitez, A.: Chlorophyll. In: Moderne Methoden der Pflanzenanalyse, K. Paech and M. V. Tracey, Eds., Vol. IV, pp. 142–196. Berlin-Göttingen-Heidelberg: Springer 1955
Takayama, K., MacLennan, D. H., Tzagoloff, A., Storner, C. D.: Studies on the electron transfer system LXVII. Polyacrylamide gelelectrophoresis of the mitochondrial electron transfer complexes. Arch. Biochem. Biophys.114, 223–230 (1964)
Takemoto, J., Lascelles, J.: Coupling between bacteriochlorophyll and membrane protein synthesis inRhodopseudomonas spheroides. Proc. nat. Acad. Sci. (Wash.)70, 799–803 (1973)
Wang, R. T., Clayton, R. K.: The absolute yield of bacteriochlorophyll fluorescence in vivo. Photochem. Photobiol.13, 215–224 (1971)
Wang, R. T., Clayton, R. K.: Isolation of photochemical reaction centres from a carotenoidles mutants ofR. rubrum. Photochem. Photobiol.17, 57–61 (1973)
Weber, K., Osborn, M.: The reliability of molecular weight determination by dodecylsulfate-polyacrylamide gel electrophoresis. J. biol. Chem.244, 4406 to 4412 (1969)
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Nieth, K.F., Drews, G. The protein patterns of intracytoplasmic membranes and reaction center particles isolated fromRhodopseudomonas capsulata . Arch. Microbiol. 96, 161–174 (1974). https://doi.org/10.1007/BF00590173
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DOI: https://doi.org/10.1007/BF00590173