Abstract
Crude extracts ofPhysarum polycephalum contain five DNA degrading enzyme activities. One enzyme activity degrades native DNA with a maximum activity at pH 3.2. Four others degrade heat-denatured DNA and have their maximum activity at pH's 3.4, 4.0, 7.6 and 8.5 respectively. The five DNA degrading activities react in different ways to administration of divalent cations and show different stabilities towards heat inactivation or incubation conditions.
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Abbreviations
- PIPES:
-
piperazine-N,N′-bis(2-ethanesulfonic acid)
References
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Polman, B.J.J., Janssen, H.M.J. & Kuyper, C.M.A. Activities of DNA degrading enzymes in the slime moldPhysarum polycephalum . Arch. Microbiol. 96, 119–124 (1974). https://doi.org/10.1007/BF00590168
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DOI: https://doi.org/10.1007/BF00590168