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Inhibition of carbonic anhydrase in dog plasma

  • Heart, Circulation, Respiration and Blood; Environmental and Exercise Physiology
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Abstract

Using a pH stat method, we measured the activity of carbonic anhydrase (CA) from dog erythrocytes in the presence of various amounts of dog plasma. A plasma factor appeared to be able to inhibit about 86% of the total CA activity, corresponding to the relative activity of CA II. Naiodoacetate was shown to inhibit the total CA activity up to about 13%, corresponding to the relative activity of CA I. Cl− inhibited the total CA activity up to about 20%, presumably mainly through its strong influence on Ca I. It is concluded that with a degree of haemolysis of up to 3%, no appreciable plasma CA activity will occur.

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Authors and Affiliations

  1. Clinical Chemical Laboratory, Diakonessenhuis Groningen, Groningen, The Netherlands

    A. Zwart

  2. Department of Physiology, University of Groningen, Bloemsingel 10, 9712 KZ, Groningen, The Netherlands

    P. Rispens, J. Hessels & W. G. Zijlstra

Authors
  1. P. Rispens
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  2. J. Hessels
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  3. A. Zwart
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  4. W. G. Zijlstra
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Dedicated to Professor Dr. R. Brinkman on the occasion of his 90th birthday

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Rispens, P., Hessels, J., Zwart, A. et al. Inhibition of carbonic anhydrase in dog plasma. Pflugers Arch. 403, 344–347 (1985). https://doi.org/10.1007/BF00589244

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  • DOI: https://doi.org/10.1007/BF00589244

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