Abstract
A membrane enzyme reactor with simultaneous separation was investigated. Enzymes, urease and aspartase, were immobilized by a porous polytetrafluoroethylene membrane. Electrical field was applied in the medium while the reaction was carried out. Products with electrical charge could be separated through the membrane from the reaction medium as they were formed. Reaction behavior was analyzed by a simple model considering both pore-migration and reaction in the skelton of the membrane. According to the analysis the inherent reaction rate of the immobilized enzymes decreases significantly. This is probably caused by the structural variation of enzymes. For the case of urease, the change of pH inside the membrane may also cause the decrease of the reaction rate. The model analysis showed that the enzyme content in the membrane and the residence time of the substrate in the membrane governed overall extent of reaction.
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Abbreviations
- e g (dm3)−1 :
-
enzyme concentration in the membrane
- L cm:
-
membrane thickness
- K m mM:
-
Michaelis constant
- Rate mmol · min−1 · g−1 :
-
rate of product formation per unit weight of enzyme
- S mM:
-
substrate concentration
- S in mM:
-
inlet substrate concentration
- S out mM:
-
outlet substrate concentration
- u cm · min−1 :
-
migration rate
- V V:
-
voltage between the electrodes
- V m mmol · min−1 · g−1 :
-
maximum reaction rate
- X :
-
conversion
- z cm:
-
distance from the surface inside the membrane
- ɛ :
-
void fraction of the porous membrane
- χ :
-
tortuosity of the membrane
- τ min:
-
space time
References
Gvozdyak, P. I.; Mogilevidh, M. F.; Nikonenko, V. U.: Electroretention of microorganisms and biological macromolecules. Prikl. Biochem. Microbiol. 13 (1977) 295–300
Furusaki, S.; Asai, N.: Enzyme immobilization by the Coulomb force. Biotechnol. Bioeng. 25 (1983) 2209–2219
Lee, C. K.; Hong, J.: Enzyme reaction in a membrane cell coupled with electrophoresis. Ann. New York Acad. Sci. 506 (1987) 489–510
Lee, C. K.; Hong, J.: Membrane reactor coupled with electrophoresis for enzymatic production of aspartic acid. Biotechnol. Bioeng. 32 (1988) 647–654
Reithael, F. J.: Ureases. In: Boyer, P. D. (Ed.): The enzymes, vol. 3, pp. 1–20. New York: Academic Press 1971
Chaney, A. L.; Marbach, E. P.: Modified reagents for determination of urea and ammonia. Clin. Chim. 8 (1962) 130–132
Lowry, H.; Rosebrough, N. J.; Farr, A. L.; Randall, R. J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193 (1951) 265–275
Imai, M.; Furusaki, S.; Miyauchi, T.: Separation of volatile materials by gas membranes. Ind. Eng. Chem. Process Design Develop. 21 (1982) 421–426
Dixon, M.; Webb, E. L. (Eds.): Enzymes, 3rd ed., pp. 11. London: Longman 1979
Molynihan, H. J.; Wang, N. H. L.: Analysis of urea hydrolysis by immobilized urease in urea-sensing electrodes. Biotechnol. Prog. 3 (1987) 90–100
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Furusaki, S., Nozawa, T. & Nomura, S. Membrane enzyme reactor with simultaneous separation using electrophoresis. Bioprocess Engineering 5, 73–78 (1990). https://doi.org/10.1007/BF00589148
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DOI: https://doi.org/10.1007/BF00589148