Summary
A method is described for localisation of the muscle cell and myoneural junction after in vivo microelectrode registration. The glass microelectrodes were filled with saturated K4Fe(CN)6 and Na2HPO4. After registration, the area surrounding the microelectrode was fixed with acetate buffered (pH 5.6) 5% glutaraldehyde, after which gelatine solution with 0.1 M Pb(NO3)2 was applied to the area. Immediately after this, the anions were driven iontophoretically from the microelectrode with a DC while the microelectrode was simultaneously raised. A sediment formed immediately above the muscle cell, which was coloured brown 1% (NH4)2S. The gelatine muscle cell preparate was set upside down in gelatine. After this had hardened it was possible to prepare the single cell layer or single cell preparate, and finally to stain the myoneural junction involved histochemically for cholinesterase.
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Grant: Pehr Oscar Klingendahl Foundation
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Nickels, J.I. A method for localisation of the muscle cell and the motor end-plate after in vivo registration with a microelectrode. Pflugers Arch. 330, 45–50 (1971). https://doi.org/10.1007/BF00588733
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DOI: https://doi.org/10.1007/BF00588733