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Unidirectional influx of phosphate across the mucosal membrane of rabbit small intestine

  • Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands
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Abstract

The influx of phosphate across the mucosal border of different regions of rabbit small intestine was investigated using the technique of Schultz et al., J. Gen. Physiol.50, 1241–1260 (1967). In the duodenum, the phosphate influx consisted of two components: 1. a saturable part, inhibited competitively by the presence of arsenate in the mucosal solution and strongly dependent on the mucosal Na concentration, and 2. a Na-independent part, linearly related to the mucosal phosphate concentration. In the jejunum and the ileum, the phosphate influx was a linear function of the mucosal phosphate concentration. In these regions arsenate had no effect on the influx, supporting the idea of a diffusional transport. HgCl2 (0.5 mM) reduced the phosphate influx in the duodenum, at 140 mM Na, to the levels under Na-free conditions. The Na-independent influx was only slightly decreased by HgCl2, suggesting that this agent affects mainly the Na-dependent phosphate influx. In the ileum HgCl2 decreased the influx by about the same amount as under Na-free conditions in the duodenum. Thus, in the rabbit, the duodenum only appears to have a Na-dependent, carrier mediated phosphate transport mechanism at the mucosal membrane. In the jejunum and the ileum the phosphate uptake seems to be by simple diffusion.

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References

  1. Barone, R., Pavaux, C., Blin, P. C., Cug, P.: Atlas d'anatomie du lapin. Paris: Masson et Cie. 1973

    Google Scholar 

  2. Berner, W., Kinne, R., Murer, H.: Phosphate transport into brush-border membrane vesicles isolated from rat small intestine. Biochem. J.160, 467–474 (1976)

    Google Scholar 

  3. Bihler, I., Cybulsky, R.: Sugar transport at the basal and lateral aspect of the small intestinal cell. Biochim. Biophys. Acta298, 429–437 (1973)

    Google Scholar 

  4. Esposito, G., Csáky, T. Z.: Extracellular space in the epithelium of rats' small intestine. Am. J. Physiol.226, 50–55 (1974)

    Google Scholar 

  5. Frizzell, R. A., Schultz, S. G.: Effects of monovalent cations on the sodium-alanine interaction in rabbit ileum. J. Gen. Physiol.56, 462–490 (1970)

    Google Scholar 

  6. Fuchs, R., Peterlik, M.: Pathways of phosphate transport in chick jejunum. Influence of vitamin D and extracellular sodium. Pflügers Arch.381, 217–222 (1979)

    Google Scholar 

  7. Ginsburg, J. M., Lotspeich, W. D.: Interrelations of arsenate and phosphate transport in the dog kidney. Am. J. Physiol.205, 707–714 (1963)

    Google Scholar 

  8. Harms, V., Stirling, C. E.: Transport of purine nucleotides and nucleosides by in vitro rabbit ileum. Am. J. Physiol.233, E47-E55 (1977)

    Google Scholar 

  9. Harrison, H. E., Harrison, H. C.: Intestinal transport of phosphate: action of vitamin D, calcium and potassium. Am. J. Physiol.201, 1007–1012 (1961)

    Google Scholar 

  10. Harrison, H. E., Harrison, H. C.: Sodium, potassium, and intestinal transort of glucose, l-tyrosine, phosphate, and calcium. Am. J. Physiol.205, 107–111 (1963)

    Google Scholar 

  11. Helbock, H. J., Forte, J. G., Saltman, P.: The mechanism of calcium transport by rat intestine. Biochim. Biophys. Acta126, 81–93 (1966)

    Google Scholar 

  12. Hoffmann, N., Thees, M., Kinne, R.: Phosphate transport by isolated renal brush border vesicles. Pflügers Arch.362, 147–156 (1976)

    Google Scholar 

  13. Kozma, C., Macklin, W., Cummins, L. M., Maurer, R.: The anatomy, physiology, and the biochemistry of the rabbit. In: The Biology of the laboratory rabbit. S. H. Weisbroth, R. E. Flatt, A. L. Kraus, eds.), pp. 50–72. New York: Academic Press 1974

    Google Scholar 

  14. McHardy, G. J. R., Parsons, D. S.: The absorption of inorganic phosphate from the small intestine of the rat. Quart. J. Exp. Physiol.41, 398–409 (1956)

    Google Scholar 

  15. Peterlik, M.: Phosphate transport by embryonic chick duodenum. Stimulation by vitamin D3. Biochim. Biophys. Acta514, 164–171 (1978)

    Google Scholar 

  16. Robinson, J. W. L.: Comparative aspects of the response of the intestine to its ionic environment. Comp. Biochem. Physiol.34, 641–655 (1970)

    Google Scholar 

  17. Rose, R. C., Schultz, S. G.: Studies on the electrical potential profile across rabbit ileum. Effects of sugars and amino acids on transmural and transmucosal electrical potential differences. J. Gen. Physiol.57, 639–663 (1971)

    Google Scholar 

  18. Rose, R. C., Nahrwold, D. L., Koch, M. J.: Electrical potential profile in rabbit ileum: role of rheogenic Na transport. Am. J. Physiol.232, E5-E12 (1977)

    Google Scholar 

  19. Sallee, V. L., Wilson, F. A., Dietschy, J. M.: Determination of unidirectional uptake rates for lipids across the intestinal brush border. J. Lipid Res.13, 184–192 (1972)

    Google Scholar 

  20. Schultz, S. G., Curran, P. F., Chez, R. A., Fuisz, R. E.: Alanine and sodium fluxes across mucosal border of rabbit ileum. J. Gen. Physiol.50, 1241–1260 (1967)

    Google Scholar 

  21. Stirling, C. E.: Mercurial perturbation of brush border membrane permeability in rabbit ileum. J. Membr. Biol.23, 33–56 (1975)

    Google Scholar 

  22. Stoll, R., Kinne, R., Murer, H., Fleisch, H., Bonjour, J.-P.: Phosphate transport by rat renal brush border membrane vesicles: Influence of dietary phosphate, thyroparathyroidectomy, and 1,25-dihydroxyvitamin D3. Pflügers Arch.380, 47–52 (1979)

    Google Scholar 

  23. Tai, Y. H., Desjeux, J. F., Danisi, G., Curran, P. F.: Na and Cl transport and short-circuit current in rabbit ileum. J. Membr. Biol.31, 189–208 (1977)

    Google Scholar 

  24. Walling, M. W.: Intestinal Ca and phosphate transport: differential responses to vitamin D3 metabolites. Am. J. Physiol.233, E488-E494 (1977)

    Google Scholar 

  25. Walling, M. W.: Effects of 1,25-dihydroxyvitamin D3 on active intestinal inorganic phosphate absorption. In: Vitamin D, biochemical, chemical and clinical aspects related to calcium metabolism (A. W. Norman, K. Schaefer, J. W. Coburn, H. F. De Luca, D. Frazer, H. G. Grigoleit, D. v. Herrath, eds.), pp. 321–330. Berlin: de Gruyter 1977

    Google Scholar 

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Danisi, G., Straub, R.W. Unidirectional influx of phosphate across the mucosal membrane of rabbit small intestine. Pflugers Arch. 385, 117–122 (1980). https://doi.org/10.1007/BF00588690

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