Novel seed lipid phenotypes in combinations of mutants altered in fatty acid biosynthesis inArabidopsis
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The seed fatty acid (FA) composition of various single mutant combinations ofArabidopsis thaliana that affect FA biosynthesis has been examined. Double mutant combinations offae, a mutation affecting CIS elongation, and a series of four other FA biosynthetic mutants were synthesized. The four other single mutants were:fad2 andfad3, which are deficient in 18∶1 and 18∶2 desaturation, respectively;fab1, which is elevated in 16∶0 and decreased in 18∶1; andfab2, which is elevated in 18∶0 and decreased in 18∶1. The superimposition of two blocks in the FA biosynthetic pathway leads to dramatic changes in the FA content of the double mutants. The tenArabidopsis stocks analyzed to date (wild-type, five single mutants, and four double mutants) make seed oils with a wide range of FA compositions, and illustrate the diversity of oils it is possible to obtain from a single plant species.
Key wordsArabidopsis thaliana Lipid biosynthesis Fatty acid desaturation Fatty acid elongation Oilseed breeding
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