Novel seed lipid phenotypes in combinations of mutants altered in fatty acid biosynthesis inArabidopsis
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The seed fatty acid (FA) composition of various single mutant combinations ofArabidopsis thaliana that affect FA biosynthesis has been examined. Double mutant combinations offae, a mutation affecting CIS elongation, and a series of four other FA biosynthetic mutants were synthesized. The four other single mutants were:fad2 andfad3, which are deficient in 18∶1 and 18∶2 desaturation, respectively;fab1, which is elevated in 16∶0 and decreased in 18∶1; andfab2, which is elevated in 18∶0 and decreased in 18∶1. The superimposition of two blocks in the FA biosynthetic pathway leads to dramatic changes in the FA content of the double mutants. The tenArabidopsis stocks analyzed to date (wild-type, five single mutants, and four double mutants) make seed oils with a wide range of FA compositions, and illustrate the diversity of oils it is possible to obtain from a single plant species.
Key wordsArabidopsis thaliana Lipid biosynthesis Fatty acid desaturation Fatty acid elongation Oilseed breeding
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- Bubeck DM, Fehr WR, Hammond EG (1989) Inheritance of palmitic and stearic acid mutants of soybean. Crop Sci 29:652–656Google Scholar
- Green AG, Marshall DR (1984) Isolation of induced mutants in linseed (Linum usitatissimum) having reduced linolenic acid content. Euphytica 33:321–328Google Scholar
- James DW, Dooner HK (1990) Isolation of EMS-induced mutants inArabidopsis altered in seed fatty acid composition. Theor Appl Genet 80:241–245Google Scholar
- Johnson RW, Fritz E (1989) Fatty acids in industry. Marcel Dekker, New York, 659 ppGoogle Scholar
- Krol AR van der, Mol JNM, Stuitje AR (1988) Modification of eukaryotic gene expression by complementary RNA or DNA sequences. BioTechniques 6:958–967Google Scholar
- Krol AR van der, Mur LA, Beld M, Mol JMN, Stuitje AR (1990) Flavonoid genes in petunia: addition of a limited number of gene copies may lead to a suppression of gene expression. Plant Cell 2:291–299Google Scholar
- Kumar PR, Tsunoda S (1980) Variation in oil content and fatty acid composition among seeds from the Cruciferae. In: Tsunoda S, Hinata K, Gomez-Campo C (eds)Brassica crops and wild allies. Japan Scientific Societies Press, Tokyo, pp 235–252Google Scholar
- Lemieux B, Miquel M, Somerville C, Browse J (1990) Mutants ofArabidopsis with alterations in seed lipid fatty acid composition. Theor Appl Genet 80:234–240Google Scholar
- Napoli C, Lemieux C, Jorgensen R (1990) Introduction of a chimeric chalcone synthase gene into petunia results in reversible co-suppression of homologous genesin trans. Plant Cell 2:279–289Google Scholar
- Stefansson BR, Hougen FW, Downey RK (1961) Note on the isolation of rape plants with seed oil free from erucic acid. Can J Plant Sci 41:218–219Google Scholar