Theoretical and Applied Genetics

, Volume 82, Issue 4, pp 409–412 | Cite as

Novel seed lipid phenotypes in combinations of mutants altered in fatty acid biosynthesis inArabidopsis

  • D. W. JamesJr.
  • H. K. Dooner


The seed fatty acid (FA) composition of various single mutant combinations ofArabidopsis thaliana that affect FA biosynthesis has been examined. Double mutant combinations offae, a mutation affecting CIS elongation, and a series of four other FA biosynthetic mutants were synthesized. The four other single mutants were:fad2 andfad3, which are deficient in 18∶1 and 18∶2 desaturation, respectively;fab1, which is elevated in 16∶0 and decreased in 18∶1; andfab2, which is elevated in 18∶0 and decreased in 18∶1. The superimposition of two blocks in the FA biosynthetic pathway leads to dramatic changes in the FA content of the double mutants. The tenArabidopsis stocks analyzed to date (wild-type, five single mutants, and four double mutants) make seed oils with a wide range of FA compositions, and illustrate the diversity of oils it is possible to obtain from a single plant species.

Key words

Arabidopsis thaliana Lipid biosynthesis Fatty acid desaturation Fatty acid elongation Oilseed breeding 


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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • D. W. JamesJr.
    • 1
  • H. K. Dooner
    • 1
  1. 1.DNA Plant TechnologyOaklandUSA

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