Pflügers Archiv

, Volume 332, Issue 4, pp 302–312 | Cite as

The isolated frog skin epithelium: Permeability characteristics and responsiveness to oxytocin, cyclic AMP and theophylline

  • R. M. Rajerison
  • M. Montegut
  • S. Jard
  • F. Morel
Article

Summary

The combined treatment of the frog skin with collagenase and hydrostatic pressure enables complete separation of the epithelial layer from the supporting dermis. The separation entirely preserves the epithelium's passive permeability and active sodium transport capacity. The short circuit current, d.c. resistance, unidirectional fluxes of Na+ and Cl ions, and osmotic water permeability were found identical on series of isolated epitheliums and intact skins obtained from the same groups of animals. Furthermore, the isolated epithelium is fully responsive to oxytocin, cyclic AMP and theophylline—three agents known to enhance active sodium transport and water permeability of the intact skin.

Key words

Frog Skin Isolated Epithelium Sodium Transport Water Permeability Oxytocin Cyclic AMP Theophylline 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Aceves, J., Erlij, D.: Sodium transport across the isolated epithelium of the frog skin. J. Physiol. (Lond.)212, 195–210 (1971).Google Scholar
  2. 2.
    Baba, W., Smith, A., Townsend, M.: The effect of vasopressin, theophylline and 3′5′ adenosine monophosphate (cyclic AMP) on sodium transport across the frog skin. Quart. J. exp. Physiol.52, 416 (1967).Google Scholar
  3. 3.
    Bastide, F., Jard, S.: Action de la noradrénaline et de l'ocytocine sur le transport actif de sodium et la perméabilité à l'eau de la peau de grenouille. Biochim. biophys. Acta (Amst.)150, 113 (1968).Google Scholar
  4. 4.
    Bourguet, J., Jard, S.: Un dispositif automatique d'enregistrement du flux net d'eau à travers la peau et la vessie des amphibiens. Biochim. biophys. Acta (Amst.)43, 182 (1966).Google Scholar
  5. 5.
    Carasso, N., Favard, P., Jard, S., Rajerison, R.: The islated frog skin epithelium: I. Preparation and general structure in different physiological states. J. Microscop.10, 315–330 (1971).Google Scholar
  6. 6.
    Cuthbert, A. W., Painter, E.: Independant action of antidiuretic hormone, theophylline and cyclic 3′5′ adenosine monophosphate on cell membrane permeability in frog skin. J. Physiol. (Lond.)199, 593 (1969).Google Scholar
  7. 7.
    Erlij, D., Aceves, J.: Sodium transport across the isolated epithelium of the frog skin. Biophys. J.9, A 163 (1969).Google Scholar
  8. 8.
    Fuhrman, F. A., Ussing, H. H.: A characteristic response of the isolated frog skin potential to neurohypophyseal principles and its relations to the transport of sodium and water. J. Cell. comp. Physiol.38, 109 (1951).Google Scholar
  9. 9.
    Morel, F., Maetz, J.: Emploi de méthodes biophysiques pour l'étude du mécanisme d'action de certaines hormones. Colloque de biophysique. Journées médicales de Bordeaux. Sté Edit. de l'enseignement supérieur (1958).Google Scholar
  10. 10.
    —, Odier, M., Lucarain, C.: Un dispositif de mesures multiples et simultanées du transport actif de sodium à travers plusieurs régions d'une même peau de grenouille. J. Physiol. (Paris)53, 757 (1961).Google Scholar
  11. 11.
    Parisi, M., Ripoche, P., Bourguet, J.: Une préparation de l'épithélium isolé de la vessie de grenouille. Réponses à l'oxytocine, au 3′5′ adenosine monophosphate cyclique, à la théophylline. Pflügers Arch.309, 59 (1969).Google Scholar
  12. 12.
    Rawlins, F., Mateu, L., Fragachan, F., Whittembury, G.: Isolated toad skin epithelium. Transport characteristics. Pflügers Arch.316, 64 (1970).Google Scholar
  13. 13.
    Rider, J., Thomas, S.: The effect of theophylline on sodium transport across frog skin in the absence of chloride. Brit. J. Pharmacol.37, 539 (1969).Google Scholar
  14. 14.
    Ussing, H. H.: Transport of electrolytes and water across epithelia. Harvey Lect.59, 1 (1963).Google Scholar
  15. 15.
    —, Zerahn, K.: Active transport of sodium as the source of electric current in the short circuited isolated frog skin. Acta physiol. scand.23, 110 (1951).Google Scholar

Copyright information

© Springer-Verlag 1972

Authors and Affiliations

  • R. M. Rajerison
    • 1
  • M. Montegut
    • 1
  • S. Jard
    • 1
  • F. Morel
    • 1
  1. 1.Laboratoire de Physiologie CellulaireCollège de FranceParisFrance

Personalised recommendations