Summary
The role of the Na concentration of the tubular fluid at the macula densa in the control of renin secretion was studied. Fifteen minutes following the intravenous administration of ethacrynic acid (2.5 mg/kg) to mongrel dogs (Group I), mean Na excretion increased from 13.7 to 449 μEq/min and mean PRA rose from 557 to 1321 ng/100 ml. When 20% mannitol was infused (5 ml/min over 30 min) (Group II), mean Na excretion increased from 9.4 to 43 μEq/min while mean PRA dropped from 630 to 445 ng/100 ml. In dogs that received ethacrynic acid and 5% dextrose (5 ml/min over 30 min) (Group III) to prevent plasma volume contraction, mean urine Na excretion increased from 16.4 to 417 μEq/min and mean PRA rose from 560 to 1540 ng/100 ml. Furthermore, 60 min following ethacrynic acid, (Groups I and III), mean Na excretion dropped to 240 and 123 μEq/min respectively, while mean PRA did not change significantly. In Group II, mean Na excretion increased slightly to 58.4 μEq/min while mean PRA rose to control levels. These data suggest that renin secretion is stimulated by an increase in Na concentration at the macula densa.
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Boucher, R., Veyrat, R., De Champlain, J., Genest, J.: New procedures for measurement of human plasma angiotensin and renin activity levels. Canad. med. Ass. J.90, 194–200 (1964).
Brown, T. C., Davis, J. O., Johnson, C. I.: Acute responses in plasma renin and aldosterone secretion to diuretics. Amer. J. Physiol.211, 437–441 (1966).
Cooke, C. R., Brown, T. C., Zacherle, B. J., Walker, W. G.: The effect of altered sodium concentration in the distal nephron segments on renin release. J. clin. Invest.49, 1630–1638 (1970).
Curran, P. F., Hajjar, J. J., Glynn, I. M.: The sodium-alanine interaction in rabbit ileum. Effect of alanine on sodium fluxes. J. gen. Physiol.55, 297–308 (1970).
Early, L. E., Friedler, R. M.: Renal tubular effects of ethacrynic acid. J. clin. Invest.43, 1495–1506 (1964).
Goldberg, M., McCurdy, D. K., Foltz, E. L., Bluemle, L. W., Jr.: Effects of ethacrynic acid (a new saluretic agent) on renal diluting and concentrating mechanisms: evidence for site of action in the loop of Henle. J. clin. Invest.43, 201–216 (1964).
Hess, R., Gross, F.: Glucose-6-phosphate dehydrogenase and renin in kidneys of hypertensive or adrenalectomized rats. Amer. J. Physiol.197, 869–872 (1959).
Hooke, J. B., Williamson, H. E.: Lack of correlation between natriuretic activity and inhibition of renal Na-K-activated ATPase. Proc. Soc. exp. Biol. (N. Y.)120, 358–360 (1965).
Imbs, J. L., Velly, J., Fontaine, J. F., Schwartz, J.: Contrôle de la secretion de renin. Nephron7, 499–511 (1970).
McNaye, J. L., Abe, Y.: Redistribution of cortical blood flow during renal vasodilation in dogs. Circulat. Res.27, 1023–1032 (1970).
Meyer, P., Menard, J., Papanicolaou, N., Alexandre, J. M., Devaux, C., Milliez, P.: Mechanism of renin release following furosemide diuresis. Amer. J. Physiol.215, 908–915 (1968).
Meyer, P., Weil, B., Menard, J., Alexandre, J. M., Devaux, C., Milliez, P.: Renin stimulation mechanism in man during induced natriuresis. Rev. Canad. Biol.27, 21–28 (1968).
Nash, F. D., Rastorfer, H. H., Bailie, M. D., Waltren, R. L., Schneider, E. G.: Renin release: relation to renal sodium load and dissociation from hemodynamic changes. Circulat. Res.22, 473–487 (1968).
Seldin, D. W., Eknoyan, G., Suki, W. N., Rector, F. C., Jr.: Localization of diuretic action from the pattern of water and electrolyte excretion. Ann. N.Y. Acad. Sci.139, 328–343 (1966).
Stein, J. H., Ferris, T. F., Huprich, J. E., Smith, T. C., Osgood, R. W.: Effect of renal vasodilatation on the distribution of cortical blood flow in the kidney of the dog. J. clin. Invest.50, 1429–1438 (1971).
Sullivan, L. P., Tucker, J. M., Scherbenske, M. J.: Effect of furosemide on sodium transport and metabolism in toad bladder. Amer. J. Physiol.220, 1316–1324 (1971).
Thurau, K.: Influence of sodium concentration at macula densa cells on tubular sodium load. Ann. N.Y. Acad. Sci.139, 388–399 (1967).
Thurau, K., Schnermann, J., Nagel, W., Horster, M., Wahl, M.: Composition of tubular fluid in the macula densa segment as a factor regulating the function of the juxtaglomerular apparatus. Circulat. Res.21 (Suppl. II), 79–89 (1967).
Ulrich, K. J., Schmidt-Nielsen, B., O'Dell, R., Pehling, G., Gottschalk, C. W., Lassiter, W. E., Mylle, M.: Micropuncture study of composition of proximal and distal tubular fluid in rat kidney. Amer. J. Physiol.204, 527–531 (1963).
Vander, A. J.: Nature of the stimulus for renin secretion in anesthetized dogs. Hypertension13, 126–130 (1965).
Vander, A. J.: Control of renin release. Physiol. Rev.47, 359–382 (1967).
Vander, A. J.: Renin secretion during mannitol diuresis and ureteral occlusion. Proc. Soc. exp. Biol. (N.Y.)128, 518–520 (1968).
Vander, A. J., Carlson, J.: Mechanism of the effects of furosemide on renin secretion in anesthetized dogs. Circulat. Res.25, 145–152 (1969).
Vander, A. J., Miller, R.: Control of renin secretion in the anesthetized dog. Amer. J. Physiol.207, 537–546 (1964).
White, F. N.: Control of renin secretion in the dog. Circulation32 (Suppl. II), 219 (1965) (Abstract).
Whittembury, G., Fishman, J.: Relation between cell Na extrusion and transtubular absorption in the perfused toad kidney: the effect of K, ouabain and ethacrynic acid. Europ. J. Physiol.307, 138–153 (1969).
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Birbari, A. Intrarenal factors in the control of renin secretion. Pflügers Arch. 337, 29–37 (1972). https://doi.org/10.1007/BF00587869
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DOI: https://doi.org/10.1007/BF00587869