Pflügers Archiv

, Volume 396, Issue 3, pp 263–264 | Cite as

A simple method for the simultaneous measurement of sodium and chloride fluxes across transporting epithelia

  • Ian G. Thompson
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands Letter and Note

Abstract

A simple tecynique for the separation of22Na and35Cl radioactivity in the same solution is described. Two samples were taken from a solution containing both22Na and36Cl. One sample was precipitated with AgNO3 and the other was untreated. Samples were assayed for radioactivity by liquid scintillation spectrophotometry. Precipitation with AgNO3 removed 100% of the36Cl activity from solution while having no effect on the22Na radioactivity. Thus precipitated samples contained only22Na radioactivity. Subtraction of the radioactivity in the supernatants of precipitated samples (i. e.22Na) from the total radioactivity of untreated samples (i. e.22Na &36Cl) yielded the level of36Cl activity in these samples.

Key words

22Na 36Cl AgNO3 precipitation ion fluxes 

References

  1. 1.
    Field, M., D. Fromm, and I. McColl, 1971. Ion Transport in Rabbit Ileal Mucosa. I. Na and Cl Fluxes and Short-circuit Current. Am. J. Physiol. 220: 1388–1396.Google Scholar
  2. 2.
    Tomlinson, R.W.S. and A.W. Wood, 1974. A method for Measuring Simultaneously the Bidirectional Naü and Cl Fluxes Across a Membrane. J. Physiol. (London) 242:57p-58p.Google Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Ian G. Thompson
    • 1
  1. 1.Renal Unit, Department of MedicineMassachusetts General HospitalBostonUSA

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