Pflügers Archiv

, Volume 342, Issue 2, pp 111–123 | Cite as

Radioactive microsphere distribution and single glomerular blood flow in the normal rabbit kidney

  • Lise Bankir
  • Nicolette Farman
  • Jean-Pierre Grünfeld
  • Edith Huet de la Tour
  • Jean-Louis Funck-Brentano
Article

Summary

Intracortical distribution of blood flow was studied in the rabbit kidney with 15 μm labelled microspheres (M) injected into the left ventricule. M injection did not alter renal function. Thanks to arterial filling of left kidney with silicone rubber, efferent vascular patterns of the glomeruli could be precisely identified. Glomeruli of different populations were sampled by microdissection and their radioactivity measured. Assuming that intracortical distribution of M reflected distribution of flow to the glomeruli, individual glomerular blood flows (GBF) were determined. In hydropenic rabbits, GBF was higher in deep glomeruli providing vasa recta (G4) (193±14 nl·min−1) than in most other superficial (G1 and G2) and deep glomeruli (G3) (190±27, 113±10 and 127±9 nl·min−1 respectively;\(\bar m \pm SEM, n = 6)\). The exact significance of GBF found in superficial glomeruli with straight ascending efferent arterioles supplying aglomerular suscapsular cortex (G1) was questioned because of the possible axial streaming of spheres. Afferent medulary blood flow was calculated to represent 9.0±0.9% of total renal blood flow.

Key words

Intrarenal Blood Flow Distribution Glomerular Blood Flow Nephron Heterogeneity Radioactive Microspheres Renal Cortex 

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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • Lise Bankir
    • 1
  • Nicolette Farman
    • 1
  • Jean-Pierre Grünfeld
    • 1
  • Edith Huet de la Tour
    • 1
  • Jean-Louis Funck-Brentano
    • 1
  1. 1.I.N.S.E.R.M. - Unité 90 Hôpital NeckerParisFrance

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