Skip to main content
SpringerLink
Log in

Relaxation of coronary arteries by electro-mechanical decoupling or adrenergic stimulation

  • Published:
Pflügers Archiv Aims and scope Submit manuscript

Summary

The contractions of isolated spiral strips from bovine and porcine coronary arteries were induced by potassium depolarization. The preparations were relaxed by verapamil and/or isoproterenol.

  1. 1.

    The non-activated preparation of bovine artery has a basal tone corresponding to 15±1.7% of the maximum of developed tension.

  2. 2.

    The contraction induced by potassium depolarization was largely prevented by 11.3 μg/ml of verapamil. In this preparation, however, 2.8 μg/ml of noradrenaline relaxed the depolarized spiral strip to the same degree as compared with values of the polarized arteries.

  3. 3.

    The depolarized, activated preparation is more relaxed by inhibition of calcium ion influx (verapamil) than by activation of beta-adrenergic receptors (isoproterenol). The relaxing effect takes place in the following order: noradrenaline (51.7%) < isoproterenol (86.1%) < verapamil (100%).

  4. 4.

    The polarized, non-activated preparation is more relaxed by stimulation of beta-adrenergic receptors (isoproterenol) than by inhibition of calcium ion influx (verapamil). The relaxing effect takes place in the following order: verapamil (64.4%) < noradrenaline (85.3%) < isoproterenol (100%).

  5. 5.

    The degree of maximal relaxation after verapamil, noradrenaline, or isoproterenol increases in the polarized preparation with augmented fibre stretch.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Berne, R. M.: Effect of epinephrine and norepinephrine on coronary circulation. Circulat. Res.6, 644–655 (1958).

    Google Scholar 

  2. Bohr, D. F.: Adrenergic receptors in coronary arteries. Ann. N. Y. Acad. Sci.139, 799–807 (1967).

    Google Scholar 

  3. Breemen, C. van, Lesser, P.: The absence of increased membrane calcium permeability during norepinephrine stimulation of arterial smooth muscle. Microvasc. Res.3, 113–114 (1971).

    Google Scholar 

  4. Bülbring, E.: Correlation between membrane potential, spike discharge and tension in smooth muscle. J. Physiol. (Lond.)128, 200–221 (1955).

    Google Scholar 

  5. Deck, K. A.: Änderungen des Ruhepotentials und der Kabeleigenschaften der Purkinje-Fäden bei der Dehnung. Pflügers Arch. ges. Physiol.280, 131–140 (1964).

    Google Scholar 

  6. Denison, A. B., Bardhanabaedya, S., Green, H. D.: Adrenergic drugs and blockade on coronary arterioles and myocardial contraction. Circulat. Res.4, 653–658 (1956).

    Google Scholar 

  7. Doutheil, U., Ensslin, A.: Wirkung von Brenzcatechinaminen auf die Coronardurchblutung an asystolischen Hundeherzen. Pflügers Arch. ges. Physiol.287, 111–123 (1966).

    Google Scholar 

  8. Doutheil, U., Ten Bruggencate, H. G., Kramer, K.: Coronarvasomotorik unter l-Noradrenalin und Isopropylnoradrenalin nach Blockierung der adrenergischen β-Receptoren durch Nethalide. Pflügers Arch. ges. Physiol.281, 181–190 (1964).

    Google Scholar 

  9. Feinberg, H., Katz, L. N.: Effect of catecholamines, l-epinephrine and l-norepinephrine on coronary flow and oxygen metabolism of the myocardium. Amer. J. Physiol.193, 151–156 (1958).

    Google Scholar 

  10. Fleckenstein, A., Döring, H. J., Kammermeier, H.: Einfluß von beta-Rezeptorenblockern und verwandten Substanzen auf Erregung, Kontraktion und Energiestoffwechsel der Myokardfaser. Klin. Wschr.46, 343–351 (1968).

    Google Scholar 

  11. Fleckenstein, A., Grün, G., Tritthart, H., Byon, K., Harding, P.: Uterus-Relaxation durch hoch aktive Ca++-antagonistische Hemmstoffe der elektromechanischen Koppelung wie Isoptin (Verapamil, Iproveratril), Substanz D 600 und Segontin (Prenylamin). Klin. Wschr.49, 32–41 (1971).

    Google Scholar 

  12. Fleckenstein, A., Kammermeier, H., Döring, H. J., Freund, H. J., Grün, G., Kienle, A.: Zum Wirkungsmechanismus neuartiger Koronardilatatoren mit gleichzeitig Sauerstoff-einsparenden Myokardeffekten, Prenylamin und Iproveratril. 1. Teil und 2. Teil, Z. Kreisl.-Forsch.56, 716–744, 839–858 (1967).

    Google Scholar 

  13. Folkow, B., Häggendahl, J., Lisander, B.: Extent of release and elimination of noradrenaline at peripheral adrenergic nerve terminals. Acta physiol. scand.72, Suppl. 307 (1967).

    Google Scholar 

  14. Glomstein, A., Hauge, A., Øye, I., Sinclair, D.: Effects of adrenaline on coronary flow in isolated perfused rat hearts. Acta physiol. scand.69, 102–110 (1967).

    Google Scholar 

  15. Grün, G., Fleckenstein, A.: Die elektromechanische Entkopplung der glatten Gefäßmuskulatur als Grundprinzip der Coronardilatation durch 4-(2′-Nitrophenyl)-2,6-dimethyl-1,4-dihydropyridin-3,5-dicarbonsäure-dimethylester (BAY a 1040, Nifepidine). Arzneimittel-Forsch. (Drug Res.)22, 334–344 (1972).

    Google Scholar 

  16. Haeusler, G.: The effect of verapamil on the contractility of smooth muscle and on excitation-secretion coupling in adrenergic nerve terminals. Angiolog.8, 156–160 (1971).

    Google Scholar 

  17. Henrich, H., Lutz, J.: Das vasculäre Escape-Phänomen am Intestinalkreislauf und seine Auslösung durch unterschiedliche vasoconstrictorische Substanzen. Pflügers Arch.329, 82–94 (1971).

    Google Scholar 

  18. Hirche, Hj.: Die Wirkung von Isoproterenol, Adrenalin, Noradrenalin und Adenosin auf die Durchblutung und den O2-Verbrauch des Herzmuskels vor und nach Blockierung der ß-Receptoren. Pflügers Arch. ges. Physiol.288, 162–185 (1966).

    Google Scholar 

  19. Holtz, P., Palm, D.: Brenzcatechinamine und andere sympathicomimetische Amine. Biosynthese und Inaktivierung. Freisetzung und Wirkung. Ergebn. Physiol.58, 1–580 (1966).

    Google Scholar 

  20. Katz, A. M.: Contractile proteins of the heart. Physiol. Rev.50, 63–158 (1970).

    Google Scholar 

  21. Lewis, F. B., Coffman, J. D., Gregg, D. E.: Effect of heart rate and intracoronary isoproterenol, levarterenol, and epinephrine on coronary flow and resistance. Circulat. Res.9, 89–95 (1961).

    Google Scholar 

  22. Ljung, B.: Use of partial alpha-receptor blockade for estimation of transmitter concentration at vasoconstrictor nerve endings. Acta physiol. scand.73, 6A (1968).

    Google Scholar 

  23. Lochner, W., Herz. In: Physiologie des Kreislaufes, Bd. 1, Edit. E. Bauereisen. Berlin-Heidelberg-New York: Springer 1971.

    Google Scholar 

  24. Luebs, E. D., Cohen, A., Zaleski, E., Bing, R. J.: The effect of nitroglycerin, intensin, isoptin, and papaverin on coronary blood flow in man, as measured by the coincidence counting technique and rubidium84. Amer. J. Cardiol.17, 535 (1966).

    Google Scholar 

  25. McRaven, D. R., Mark, A. L., Abbound, F. M., Mayer, H. E.: Responses of coronary vessels to adrenergic stimuli. J. clin. Invest.50, 773–778 (1971).

    Google Scholar 

  26. Mekata, H., Niu, H.: Electrical and mechanical responses of coronary artery smooth muscle to catecholamines. Jap. J. Physiol.19, 599–608 (1969).

    Google Scholar 

  27. Melville, K. I., Benfey, B. G.: Coronary vasodilator and cardiac adrenergic blocking effects of iproveratril. Canad. J. Physiol. Pharmacol.43, 339–342 (1965).

    Google Scholar 

  28. Mignault, J. de L.: Coronary cineangiographic study of intravenously administered isoptin. Canad. med. Ass. J.95, 1252–1253 (1966).

    Google Scholar 

  29. Murphy, R. A., Bohr, D. F., Newman, D. L.: Arterial actomyosin: Mg, Ca, and ATPion dependencies for ATPase activity. Amer. J. Physiol.217, 666–673 (1969).

    Google Scholar 

  30. Orlov, R. S., Plekhanov, I. P.: Changes of membranic potentials of smooth muscles of vessels in response to tension. Dokl. Akad. Nauk. SSR.175, 254–255 (1967).

    Google Scholar 

  31. Peiper, U., Griebel, L., Wende, W.: Activation of vascular smooth muscle of rat aorta by noradrenaline and depolarization: two different mechanisms. Pflügers Arch.330, 74–89 (1971).

    Google Scholar 

  32. Proctor, E.: The effects of physiological concentrations of noradrenaline on the coronary resistance of isolated perfused hearts of the cat, dog, and monkey. J. Pharm. Pharmacol.20, 36–40 (1968).

    Google Scholar 

  33. Rudolph, W., Kriener, J., Meister, W.: Die Wirkung von Verapamil auf Coronardurchblutung, Sauerstoffutilisation und Kohlendioxydproduktion des menschlichen Herzens. Klin. Wschr.49, 982–988 (1971).

    Google Scholar 

  34. Schmidt, E., Peiper, U.: Einfluß der Vordehnung auf die Dynamik der glatten Gefäßmuskulatur. Pflügers Arch.333, 314–325 (1972).

    Google Scholar 

  35. Schmidt, E., Peiper, U.: Tonusänderungen von isolierten Coronararterien durch Depolarisation und/oder Noradrenalin vor und nach Blockierung adrenerger Receptoren. Naunyn-Schmiedeberg's Arch. Pharmacol. (in press).

  36. Sparrow, M. P., Maxwell, L. C., Rüegg, J. C., Bohr, D. F.: Preparation and properties of a calcium ion sensitive actomyosin from arteries. Amer. J. Physiol.219, 1366–1372 (1970).

    Google Scholar 

  37. Tritthart, H., Kaufmann, R., Rost, B.: Inotrope Effekte extracellulärer Ca++-Variation an isolierten Herzmuskelzellen in Zellkulturen. Pflügers Arch.307, R 27 (1969).

    Google Scholar 

  38. Uchida, E., Bohr, D. F.: Myogenic tone in isolated perfused resistance vessels from rat. Amer. J. Physiol.216, 1343–1350 (1969).

    Google Scholar 

  39. Zuberbuhler, R. C., Bohr, D. F.: Responses of coronary smooth muscle to catecholamines. Circulat. Res.16, 431–440 (1965).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Physiologisches Institut der Universität Würzburg, Würzburg, Germany

    Ulrich Peiper & Erich Schmidt

Authors
  1. Ulrich Peiper
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Erich Schmidt
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Peiper, U., Schmidt, E. Relaxation of coronary arteries by electro-mechanical decoupling or adrenergic stimulation. Pflugers Arch. 337, 107–117 (1972). https://doi.org/10.1007/BF00587834

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00587834

Key words

Search

Navigation