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The “in vivo” and “in vitro” CO2-equilibration curves of blood during acute hypercapnia and hypocapnia

I. Experimental investigations

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Summary

The theory that the base excess (BE) of blood is influenced during short respiratory disturbances by bicarbonate exchange with the interstitial fluid was quantitatively tested. During inspiration of CO2 and during active hyperventilation of awake man measurements in arterialized ear-lobe and venous blood were made. During hypercapnia BE decreased by 0.09 meq/l per mm HgpCO2-During hypocapnia it increased by 0.06–0.08 meq/l · mm Hg, after correction for newly formed lactic acid, however, an increase of 0.09–0.10 meq/l · mm Hg was found.

For hyper- as well as for hypocapnia practically linear “in vivo” CO2-equilibration curves with similar slopes were obtained in the [H+]-pCO2 and in the pH-logpCO2 coordinate system. The equation of the common equilibration line from pH 7.25–7.75 is:

$$\log {\mathbf{ }}p{\text{CO}}_{\text{2}} = - 1.23{\text{pH + 10}}{\text{.71}}{\text{.}}$$

The slope is flatter than for blood under “in vitro” conditions.

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Authors and Affiliations

  1. Physiologisches Institut der Deutschen Sporthochschule Köln, Carl-diem-Weg, D-5000, Köln 41, Germany

    Dieter Böning, Ursula Schweigart & Volker Nutz

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  1. Dieter Böning
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  2. Ursula Schweigart
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Supported by Deutsche Forschungsgemeinschaft BO 360/1.

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Böning, D., Schweigart, U. & Nutz, V. The “in vivo” and “in vitro” CO2-equilibration curves of blood during acute hypercapnia and hypocapnia. Pflugers Arch. 350, 201–212 (1974). https://doi.org/10.1007/BF00587799

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