Summary
A plasma membrane enriched fraction (PMF) from rat kidney homogenates with a high specific activity of a Mg2+, Na+ and K+ activated ATPase was investigated. For comparison ATPase activity was measured also in the microsomal fraction. Adrenalectomy resulted in an increase of specific activities of Mg-, MgNaK- and NaK-ATPase in the PMF and a decrease of specific activities in the microsomal fraction. The increase of enzyme activities in the PMF has been demonstrated with three different techniques of ATPase preparation: with NaJ- or DOC-treatment and dialysis. The changes were more prominent when the adrenalectomized rats received tap water instead of saline as drinking fluid and thus were Na deprived. Kinetic studies revealed similarK m values for Na+, K+ and MgATP in control and adrenalectomized rats.V max values, however, increased about two-fold in the adrenalectomized animals. There was no difference in the allosteric activitation of the NaK-ATPase in both animal groups as indicated by equal “n” values.
The increase in ATPase activity could be prevented by substitution with daldosterone plus dexamethasone although the plasma concentration of Na remained unaltered low because of Na+ deprivation. Cortisone exerted no influence on ATPase activity in adrenalectomized rats.
From the data presented it is inferred that neither the activity of the ATPase enzyme system of the PMF nor that of the microsomal fraction is directly regulated by aldosterone. It is concluded that the changes observed are due to an adaptation to altered renal Na+ reabsorption which in turn is regulated by corticosteroids. This adaptive process is different in different subcellular fractions of rat tissue.
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Abbreviations
- PMF:
-
Plasma Membrane Enriched Fraction
- DOC:
-
Na-Deoxycholate
- NaK-ATPase:
-
Activation of basal Mg-ATPase by Na+ and K+ (=MgNaK-ATPase (-) Mg-ATPase)
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A preliminary report has been published by Ebelet al., 1968.
Supported by the Senate of West Berlin. Present address: Instituto Di Pathologia Speciale, Medica E Metodologia Clinica, Policlinico, Napoli.
Supported by grants of the Deutsche Forschungsgemeinschaft.
Other abbreviations have the usual connotation.
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De Santo, N.G., Ebel, H. & Hierholzer, K. Plasma cell membranes of the rat kidney. Pflugers Arch. 324, 26–42 (1971). https://doi.org/10.1007/BF00587794
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DOI: https://doi.org/10.1007/BF00587794