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Juxtaglomerular granules in subcellular fractions of guinea-pig kidney

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Summary

The preparation of a subcellular fraction of renin-containing juxtaglomerular granules of guinea-pig kidney is described. Two separate preparations had a 8.4 and 15 times higher pressor activity [3] per mg protein than the respective homogenate. Electron microscopy showed mitochondria, non-specific and specific juxtaglomerular granules in the granular fraction. It is concluded, that the concept of adult guinea-pig kidney as an agranular model for the investigation of production and release of renin has to be modified.

Zusammenfassung

Es wird eine Methode zur Gewinnung angereicherter Fraktionen juxtaglomerulärer Granula der Meerschweinchenniere beschrieben. Die Endfraktion zweier Präparationen hatte, auf den jeweiligen Eiweißgehalt bezogen, bei der Bestimmung nach Boucher [3], eine 8,7 bzw. 15 mal höhere pressorische Aktivität als das Ausgangshomogenat. Die elektronenmikroskopische Untersuchung zeigte neben Mitochondrien und unspezifischen auch spezifische Renin-haltige Granula. Aus diesen Befunden ergibt sich, daß die Nieren erwachsener Meerschweinchen entgegen früheren Annahmen kein besonderes granulafreies Modell zum Studium der Synthese und Freisetzung von Renin sind.

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References

  1. Biava, C., West, M.: Fine morphology of normal juxtaglomerular cells: II: Specific and non-specific cytoplasmic granules. Amer. J. Path.49, 955 (1966).

    Google Scholar 

  2. ——: Ultrastructural observations on the morphogenesis of non-specific granules in human juxtaglomerular and renal vascular cells. Circulat. Res., Suppl.II, 47,20/21 (1967).

    Google Scholar 

  3. Boucher, R., Menard, J., Genest, J.: A micromethod for measurement of renin in plasma and kidney of rats. Canad. J. Physiol. Pharmacol.45, 881 (1967).

    Google Scholar 

  4. Bucher, O., Reale, E.: Zur elektronenmikroskopischen Untersuchung der juxtaglomerulären Spezialeinrichtungen der Niere. III. Die epitheloiden Zellen der Arteriola afferens. Z. Zellforsch.56, 344 (1962).

    Google Scholar 

  5. de Duve, C.: The Lysosome. Scientific American 2 (May 1963).

  6. Janigan, D. T.: Fluorochrome staining of juxtaglomerular cell granules. Arch. Path.79, 370 (1965).

    Google Scholar 

  7. Luft, J. H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol.9, 409 (1961).

    Google Scholar 

  8. Millonig, G.: Advantages of a phosphate buffer for OsO4 solutions in fixation. J. appl. Physiol.32, 1637 (1961).

    Google Scholar 

  9. Oberling, C.: Further studies on the preglomerular cellular apparatus. Amer. J. Path.20, 155 (1944).

    Google Scholar 

  10. Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol.17, 208 (1963).

    Google Scholar 

  11. Riedel, B., Bucher, O.: Die Ultrastruktur des juxtaglomerulären Apparates des Meerschweinchens. Z. Zellforsch.79, 244 (1967).

    Google Scholar 

  12. Schaechtelin, G., Riedel, B.: Reninaktivität in Nieren ohne “Renin-Granula”. Naunyn-Schmiedebergs Arch. Pharmak. exp. Path.255, 65 (1966).

    Google Scholar 

  13. Schaffenberg, L. A., Haas, E., Goldblatt, H.: Concentration of renin in kidneys and angiotensinogen in serum of various species. Amer. J. Physiol.199, 788 (1960).

    Google Scholar 

  14. Schmidt, D., Karsunky, K.-P., Schneider, D., Soell, G., Taugner, R.: Struktur und Funktion isolierter Renin-haltiger Granula aus Schweinenieren. Gemeinsame Tagung der Italienischen und Deutschen Pharmakologischen Gesellschaften, Heidelberg, 27.–30. September 1970. Naunyn-Schmiedebergs Arch. Pharmak.269, 487 (1971).

    Google Scholar 

  15. —, Soell, G., Karsunky, K.-P., Taugner, R.: Präparation und ATPase-Aktivität reninhaltiger Granula. Experientia (Basel)27, 137 (1971).

    Google Scholar 

  16. Smith, C. L.: Rapid demonstration of juxtaglomerular granules in mammals and birds. Stain Technol.41, 291 (1966).

    Google Scholar 

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Authors and Affiliations

  1. I. Physiologisches Institut und Anatomisches Institut, III. Lehrstuhl der Universität Heidelberg, Akademiestr. 3, BRD-6900, Heidelberg 1, Germany

    D. Schmidt, R. Taugner & Chr Dénéréaz

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  1. D. Schmidt
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  2. R. Taugner
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  3. Chr Dénéréaz
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Additional information

This work was supported by the Deutsche Forschungsgemeinschaft.

We thank Dr. W. G. Forssmann for help and discussion.

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Schmidt, D., Taugner, R. & Dénéréaz, C. Juxtaglomerular granules in subcellular fractions of guinea-pig kidney. Pflugers Arch. 328, 221–226 (1971). https://doi.org/10.1007/BF00587732

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  • DOI: https://doi.org/10.1007/BF00587732

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