Summary
Activation of the bacteriophage T4 late gene 22 carried on the phage genome (ciscondition) or borne on a plasmid (transcondition) was quantitatively analyzed after phage infection using alacZ reporter gene. Transcription from the late promoter was activated postreplicatively by concomitantly infecting T4 phage. A truncated T4-lacZ gene including only 15 by of sequence upstream of the late promoter consensus of gene 22 TATAAATA, was fully activated in the ciscondition but induced to a reduced extent in the transcondition. A possible mechanism for trans-activation is discussed.
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Abbreviations
- bp:
-
base-pair(s)
- kbp:
-
kilobase-pair(s)
- T4dC phage:
-
cytosine-substituting T4 phage
- moi:
-
multiplicity of infection
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Noguchi, T., Takahashi, H. Activation and quantitative estimation of bacteriophage T4 late regulatory signal in cis- and transconditions. Molec. Gen. Genet. 233, 319–321 (1992). https://doi.org/10.1007/BF00587595
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DOI: https://doi.org/10.1007/BF00587595