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Molecular and General Genetics MGG

, Volume 233, Issue 1–2, pp 311–314 | Cite as

Identification of anodD-like gene inFrankia by direct complementation of aRhizobium nodD-mutant

  • Li-mei Chen
  • Yu-hai Cui
  • Min Qin
  • Yan-ling Wang
  • Xue-liang Bai
  • Qing-sheng Ma
Short communications

Summary

Clones from aFrankia At4 gene bank were pooled into groups and mass conjugated into anodD mutant ofRhizobium leguminosarum bv.viciae by triparental matings. When peas were inoculated with the pooled transconjugants, nodulation was observed. A plasmid, pAt2GX containingFrankia DNA, was isolated from bacteria recovered from these nodules. This plasmid was shown to complement anodD mutant ofR. leguminosarum bv.viciae. Thus pAt2GX contains aFrankia gene that is functionally equivalent tonodD ofR. leguminosarum bv.viciae.

Key words

Frankia Nod Nodule Complementation Mutant 

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Li-mei Chen
    • 1
  • Yu-hai Cui
    • 1
  • Min Qin
    • 1
  • Yan-ling Wang
    • 1
  • Xue-liang Bai
    • 1
  • Qing-sheng Ma
    • 1
  1. 1.Laboratory of Molecular GeneticsGuangxi Agricultural CollegeNanningChina

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