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Characterization of two circular plasmids from the marine diatomCylindrotheca fusiformis: plasmids hybridize to chloroplast and nuclear DNA

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Summary

This paper reports the discovery and initial characterization of two small plasmids, pCfl and pCf2, in the marine diatomCylindrotheca fusiformis. Extracted diatom DNA separates into two bands in CsCI-Hoechst 33258 dye gradients. Upon agarose gel electrophoresis of a sample of the upper band of the gradient we observed, in addition to high molecular weight (genomic) chloroplast and mitochondrial DNA, pairs of lower molecular weight bands. These bands contained two species of circular plasmid DNA molecules, as shown by electron microscopy. The nucleotide composition of the plasmids, and chloroplast and mitochondrial DNAs is similar, as indicated by their co-banding in the gradients. They were cloned, and their restriction maps determined, showing that pCfl is 4.27 and pCf2 4.08 kb in size. By hybridization analysis, we showed that pCfl and pCf2 share regions of similarity, but not identity. Neither plasmid hybridizes with mitochondrial DNA. Both plasmids hybridize with chloroplast DNA, and pCf2 also hybridizes with nuclear DNA.

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Author notes

  1. Jerry D. Jacobs

    Present address: Department of Zoology, University of Washington, 98195, Seattle, WA, USA

  2. James R. Ludwig

    Present address: Hybritech, Inc., 92196-9006, San Diego, CA, USA

  3. Teng-Yung Feng

    Present address: Institute of Botany, 11529, Academia Sinica, Taipei, Taiwan, Republic of China

  4. Robin W. Ord

    Present address: Ministry of Agriculture and Fisheries, Ruakura (Hamilton), New Zealand

Authors and Affiliations

  1. Scripps Institution of Oceanography, and Center for Molecular Genetics, University of California, 92093, San Diego, La Jolla, CA, USA

    Jerry D. Jacobs, James R. Ludwig, Mark Hildebrand, Allen Kukel, Teng-Yung Feng, Robin W. Ord & Benjamin E. Volcani

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  1. Jerry D. Jacobs
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  2. James R. Ludwig
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  3. Mark Hildebrand
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  4. Allen Kukel
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  6. Robin W. Ord
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  7. Benjamin E. Volcani
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Jacobs, J.D., Ludwig, J.R., Hildebrand, M. et al. Characterization of two circular plasmids from the marine diatomCylindrotheca fusiformis: plasmids hybridize to chloroplast and nuclear DNA. Molec. Gen. Genet. 233, 302–310 (1992). https://doi.org/10.1007/BF00587592

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  • DOI: https://doi.org/10.1007/BF00587592

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