Pflügers Archiv

, Volume 400, Issue 4, pp 372–376 | Cite as

Corticosteroid metabolism in isolated rat kidney in vitro

II. Sex dependency of metabolism and formation of 11-dehydro-corticosterone
  • H. Siebe
  • D. Tsiakiras
  • K. Hierholzer
  • Lichtenstein
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands


We have previously demonstrated that isolated kidneys from male rats convert corticosterone (B). The metabolites are formed in renal tissue, released into the recirculating perfusate and excreted in the urine (5). They have been identified as: 11-dehydro-20ζ-dihydro-B (met I), 20 ζ-dihydro-B (met II) and 5α-H-4,5-dihydro-B (met III), using HPLC. In view of sex dependency of hepatic corticosteroid metabolism we have presently investigated whether or not equivalent sexual differences exist in renal tissue. In applying appropriate HPLC-techniques we could demonstrate a fourth metabolite formed from B, which was chromatographically identical with 11-dehydro-B (=met IV). Female rat kidneys form predominantly the less polar metabolites III and IV, in contrast to kidneys from male rats, which produce, met III and the more polar metabolites I and II

Key words

Renal corticosteriod metabolism Corticosterone Isolated rat kidney Sex dependency of renal steroid metabolism HPLC-analysis 11-dehydro-B 











high pressure liquid chromatography(y)

met I (II, III, IV)

metabolite I (II, III, IV)




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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • H. Siebe
    • 1
  • D. Tsiakiras
    • 1
  • K. Hierholzer
    • 1
  • Lichtenstein
    • 1
  1. 1.Institut für Klinische PhysiologieKlinikum Steglitz der Freien Universität BerlinBerlin 45

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