Advertisement

Pflügers Archiv

, Volume 400, Issue 4, pp 372–376 | Cite as

Corticosteroid metabolism in isolated rat kidney in vitro

II. Sex dependency of metabolism and formation of 11-dehydro-corticosterone
  • H. Siebe
  • D. Tsiakiras
  • K. Hierholzer
  • Lichtenstein
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands

Abstract

We have previously demonstrated that isolated kidneys from male rats convert corticosterone (B). The metabolites are formed in renal tissue, released into the recirculating perfusate and excreted in the urine (5). They have been identified as: 11-dehydro-20ζ-dihydro-B (met I), 20 ζ-dihydro-B (met II) and 5α-H-4,5-dihydro-B (met III), using HPLC. In view of sex dependency of hepatic corticosteroid metabolism we have presently investigated whether or not equivalent sexual differences exist in renal tissue. In applying appropriate HPLC-techniques we could demonstrate a fourth metabolite formed from B, which was chromatographically identical with 11-dehydro-B (=met IV). Female rat kidneys form predominantly the less polar metabolites III and IV, in contrast to kidneys from male rats, which produce, met III and the more polar metabolites I and II

Key words

Renal corticosteriod metabolism Corticosterone Isolated rat kidney Sex dependency of renal steroid metabolism HPLC-analysis 11-dehydro-B 

Abbreviations

Alb

albumin

B

corticosterone

*B

(1,2,6,7-3H)-corticosterone

CS

corticosteroid

HPLC

high pressure liquid chromatography(y)

met I (II, III, IV)

metabolite I (II, III, IV)

perf

perfusate

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Refernces

  1. 1.
    Begue RJ, Gustafsson JA, Gustafsson SA (1973) Irreversible neonatal differentiation of corticosterone metabolism in rats in vivo. Eur J Biochem 40:361–366Google Scholar
  2. 2.
    Carlstedt-Duke J, Gustafsson JA, Gustafsson SA (1975) Sexual differences in hepatic metabolism and intra-cellular distribution of corticosterone studied by pulse labeling with 1,2,6,7-3H corticosterone. Biochem 14:639–648Google Scholar
  3. 3.
    Erickson RE, Ertel RJ, Ungar F (1966) Effect of SU-4885 on steroid 18-hydroxylation in the mouse adrenal in vitro. Endocrinology 78:343–349Google Scholar
  4. 4.
    Goldman AS, Gustafsson JA, Gustafsson SA (1973) Female pattern of metabolism of 4-14C corticosterone in male pseudohermaphroditic rats. Proc Soc Biol Med 142:691–696Google Scholar
  5. 5.
    Hierholzer K, Schöneshöfer M, Siebe H, Tsiakiras D, Weskamp P with technical assistance of Ingrid Lichtenstein (1984) Corticosteroid metabolism in isolated rat kidney in vitro. I. Formation of lipid soluble metabolites from corticosterone (B) in renal tissue from male rats. Pflügers Arch 400:363–371Google Scholar
  6. 6.
    Hoyer GA, Tsiakiras D, Siebe H, Hierholzer K (1984) Corticosteroid metabolism in isolated rat kidney in vitro. III. Structure analysis of lipophilic metabolites of corticosterone. Pflügers Arch 400:377–380Google Scholar
  7. 7.
    Linèt O, Lomen P (1971) Effect of androgens and anabolic steroids on the plasma disappearance curve and the distribution of (1,2-3H) corticosterone in the rat. Acta Endocrinology 68:303–310Google Scholar
  8. 8.
    Morris DJ, Berek JS, Davis RP (1972) Sex-dependence of the metabolism of aldosterone in adrenalectomized and intact rats. Steroids 21:397–407Google Scholar
  9. 9.
    Morris DJ (1981) The metabolism and mechanism of action of aldosterone. Endocrine Rev 2:234–247Google Scholar
  10. 10.
    Morris DJ, Berek JS, Davis RP (1973) The physiological response to aldosterone in adrenalectomized and intact rats and its sex dependence. Endocrinology 92:989–993Google Scholar
  11. 11.
    Reach G, Nakane H, Nakane Y, Auzan C, Corvol P (1977) Cortisol metabolism and excretion in the isolated perfused rat kidney. Steroids 30:605–619Google Scholar
  12. 12.
    Rocci ML Jr, Jusko WJ (1981) Analysis of prednisone, prednisolone and their 20 β-hydroxylated metabolites by high-performance liquid chromatography. J Chromatogr 224:221–227Google Scholar
  13. 13.
    Troop RC (1959) Influence of gonadal hormones on the metabolism of cortisone. Endocrinology 64:671–675Google Scholar
  14. 14.
    Tsiakiras D, Hoyer GA, Siebe H, Hierholzer K (1983) Renal metabolism of corticosterone (B) in vitro. Deutsche Pharmakol Ges und Deutsche Physiol Ges, Mainz. Naunyn-Schmiedeberg's Archives of Pharmacology, [Suppl] 322:R58Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • H. Siebe
    • 1
  • D. Tsiakiras
    • 1
  • K. Hierholzer
    • 1
  • Lichtenstein
    • 1
  1. 1.Institut für Klinische PhysiologieKlinikum Steglitz der Freien Universität BerlinBerlin 45

Personalised recommendations